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SNP Genotyping Analysis Using TaqMan Assays

The proven gold standard for detection and discrimination of virtually every SNP of interest

Single nucleotide polymorphisms (SNPs) are a common source of genetic variation within genomic DNA and typically confer a single nucleotide difference within a specific gene locus, resulting in two alleles of the gene. SNPs within the coding or regulatory regions of a gene can affect function, or more commonly serve as biological markers for inheritance of specific loci.

Applied Biosystems TaqMan SNP Genotyping assays help you detect and distinguish SNPs accurately and efficiently, so you can better understand their associations and roles in gene function and disease.

  • Minor Groove Binder (MGB) technology enables TaqMan probes to discriminate between highly homologous allele sequences
  • Robust design pipeline with 90+ parameters for optimal primer-probe combinations
  • Functional quality testing with 20 gDNA samples for each human assay
  • Flexible formats, including single tubes; 48-, 96-, and 384-well plates; and OpenArray plates, to accommodate any throughput and number of targets and samples
  • Gold-standard chemistry backed by more than 40,000 publications and over 300 patents
  • TaqMan Performance Guarantee ensures the assay will work as described or we’ll replace it

Each TaqMan SNP Genotyping Assay includes two differentially labeled, allele-specific TaqMan MGB probes and a PCR primer pair that uniquely amplify and provide unmatched specificity for the allele of interest. Choose an assay from one of more than 17 million predesigned assays, or customize your own using our Custom Assay Design Tool.

Leverage the power of MGB for your genotyping studies

Our MGB probes found in every TaqMan SNP Genotyping Assay offer industry-leading specificity and sensitivity and have long been considered the gold standard in qPCR. The MGB moiety at the 3ʹ end of the probe increases the melting temperature (Tm) of the probe and stabilizes probe–template hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing maximal assay design flexibility and superior discrimination between alleles differing by just one nucleotide.

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