Looking for the Right AAV Workflow?
Whether your research focuses on gene delivery, functional genomics, CRISPR editing, disease modelling, neuroscience, or preclinical studies, our specialists can help identify the most suitable Takara Bio AAV solution for your application.
Why Researchers Choose Takara Bio
- ✓ Helper-free AAV production without the use of a helper virus
- ✓ Flexible solutions for multiple AAV serotypes and research scales
- ✓ Streamlined products for extraction, concentration, purification, and titration
- ✓ Reliable AAV-based delivery for gene-function and CRISPR applications
- ✓ Integrated workflow support from vector construction to expression analysis
Products Associated with the Workflow
1. Gene Cloning & AAV Vector Design
Build recombinant AAV constructs efficiently with seamless cloning tools that support gene insertion, promoter selection, and vector engineering.
Featured Technologies
- In-Fusion® Cloning Technology
- AAV vector plasmids and control vectors
2. Helper-Free AAV Production
Generate high-titer recombinant AAV particles using optimized packaging systems that eliminate the need for a helper virus.
Featured Technologies
- AAVpro® Helper Free Systems
- AAVpro® 293T Cell Line
3. AAV Recovery & Concentration
Recover AAV particles from producer cells and reduce sample volume using streamlined solutions designed for efficient downstream processing.
Featured Technologies
- AAVpro® Extraction Solution
- AAVpro® Concentrator
4. AAV Purification
Obtain highly purified AAV particles through rapid, column-based workflows suitable for multiple serotypes and research applications.
Featured Technologies
- AAVpro® Purification Kit (All Serotypes)
- AAVpro® Purification Kit Maxi (All Serotypes)
5. AAV Titration & Quality Control
Quantify AAV vector genomes using a standardized real-time PCR workflow for consistent assessment of viral preparations.
Featured Technologies
- AAVpro® Titration Kit (for Real Time PCR) Ver.2
6. Gene Delivery & Functional Studies
Deliver genes into cultured cells or in vivo research models for gene-function analysis, disease modelling, neuroscience, and preclinical research.
Featured Technologies
- AAVpro® Helper Free Systems across selected serotypes
- AAV control vectors
7. CRISPR & Genome Editing
Enable AAV-mediated delivery of genome-editing components for functional genomics and editing studies, including difficult-to-transfect cell models.
Featured Technologies
- AAVpro® CRISPR/Cas9 Systems
- Guide-it™ CRISPR tools
8. Gene Expression & Validation
Confirm successful delivery and evaluate downstream expression using sensitive reverse transcription and real-time PCR technologies.
Featured Technologies
- PrimeScript™ RT Technology
- TB Green® qPCR Chemistry
