Reach New Peaks in Sanger Sequencing Performance

For decades, BigDye™ Terminator chemistry has been the gold standard for high-quality Sanger sequencing. Building on this legacy, the latest BigDye™ Terminator Plus and BigDye™ M13 HiRes Primers deliver greater speed, improved sequence quality, and enhanced performance on challenging templates—without disrupting the workflows laboratories already trust.

Whether you're sequencing plasmids, PCR products, or difficult genomic regions, the BigDye™ portfolio helps you generate accurate, reliable results with greater efficiency.

BigDye™ Terminator Plus

Faster Reactions. Greater Confidence. Same Trusted Workflow.

BigDye™ Terminator Plus is the newest generation of cycle sequencing chemistry, designed to improve laboratory productivity while maintaining the accuracy and reliability expected from the BigDye™ family.

Key Features & Benefits

• Up to 50% faster sequencing reactions
• Enhanced performance on GC-rich and difficult templates
• High-quality sequencing across challenging regions
• Compatible with existing BigDye™ sequencing workflows
• No protocol redesign or workflow disruption
• Reliable, reproducible sequencing performance

Optimized for Challenging Templates

Generate high-quality sequencing data from:

• GC-rich DNA
• Repetitive sequences
• Difficult PCR products
• Complex sequencing templates

Accelerate Laboratory Productivity

Shorter reaction times help laboratories:

• Reduce turnaround time
• Increase sample throughput
• Improve workflow efficiency
• Maintain high sequencing quality

Seamless Workflow Integration

BigDye™ Terminator Plus is designed to integrate effortlessly into established sequencing workflows, allowing laboratories to adopt improved chemistry without retraining personnel or revalidating protocols.

BigDye™ M13 HiRes Primers

Higher Resolution from the Very First Base

BigDye™ M13 HiRes Primers are designed to improve sequence quality at the 5′ end, enabling clearer basecalling immediately after the primer and extending high-quality read lengths where conventional primers often struggle.

Key Features & Benefits

• Enhanced 5′ end sequence resolution
• Improved early basecalling accuracy
• Better peak separation during capillary electrophoresis
• Proprietary primer chemistry
• Compatible with existing M13 sequencing workflows
• No assay redesign required

Maintain Existing Workflows

BigDye™ M13 HiRes Primers retain the standard M13 forward and reverse primer sequences, enabling straightforward adoption with existing M13-tagged templates while delivering improved sequencing performance.

Broad Compatibility

Compatible with:

• BigDye™ Terminator Plus Cycle Sequencing Kits
• BigDye™ Terminator v3.1 Cycle Sequencing Kits
• Standard capillary electrophoresis systems using POP-7 polymer

Flexible Deployment

Choose the solution that best fits your laboratory:

• Integrated with the BigDye™ Terminator Plus Kit with HiRes Primers
• Available as standalone primers for existing sequencing workflows

Why Choose the BigDye™ Portfolio?

The latest BigDye™ sequencing solutions help laboratories:

• Reduce sequencing turnaround times
• Improve data quality from challenging templates
• Achieve superior 5′ end sequence resolution
• Increase confidence in sequencing results
• Preserve existing workflows and instrument investments
• Enhance laboratory productivity without additional complexity

Whether you're performing routine Sanger sequencing or tackling complex sequencing challenges, the BigDye™ portfolio delivers the performance needed to advance your research with confidence.

Discover how the latest BigDye™ innovations can help your laboratory achieve faster, higher-quality Sanger sequencing results.