Overview
The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System for Human Identification (HID) is a compact and high-performance platform designed for precise DNA quantification in forensic workflows. Engineered to meet the stringent requirements of casework and database processing, the system delivers consistent, reproducible results when used with validated HID chemistries and analysis software.
With an intuitive interface, advanced thermal control, and multiplexing capability, it enables efficient and scalable qPCR workflows in regulated forensic laboratory environments.
Features
• Compact benchtop design with optimized footprint and front-facing motorized block for efficient lab space utilization
• Integrated touchscreen interface for simplified run setup and real-time amplification monitoring
• Bright white LED excitation source with long operational life (>5 years)
• Supports up to six-dye multiplexing for advanced quantification workflows
• VeriFlex™ Peltier block with six independent temperature zones for precise thermal control
• HID-validated compatibility with Quantifiler™ Trio, HP, Duo, and Human kits
• Onboard data storage supporting approximately 2,000–5,000 runs
Specifications
• Sample Capacity: 96-well (0.2 mL) format
• Reaction Volume: 10–100 µL
• Detection Method: Whole-plate imaging
• Excitation Source: Bright white LED
• Optical System: 6 decoupled filters (up to 21 dye combinations)
• Temperature Control: VeriFlex™ blocks with 6 independent zones
• Dimensions: 27 × 50 × 40 cm (W × D × H)
• Weight: <26 kg
• Connectivity: Cloud platform, USB, Wi-Fi, Ethernet
• Compliance: ISO 13485
Applications
• Forensic DNA quantification for human identification workflows
• Casework and database sample processing
• STR workflow preparation and normalization
• qPCR-based nucleic acid quantification in regulated environments
Technical Snapshot
• Technology: Real-time PCR (qPCR) with fluorescence detection
• Workflow: Automated amplification and real-time quantification
• Output: Quantitative DNA data with high sensitivity and reproducibility
• Use Case: Forensic and human identification laboratories requiring validated, high-confidence qPCR
