Applied Biosystems SeqStudio 8 Flex Genetic Analyzer for HID
SeqStudio™ 8 Flex Genetic Analyzer
The Applied Biosystems™ SeqStudio™ 8 Flex Genetic Analyzer for Human Identification (HID) is a mid-throughput benchtop system engineered specifically for forensic and human identification laboratories. Utilizing trusted capillary electrophoresis (CE) technology, it delivers Sanger sequencing and fragment analysis data tailored for the rigorous demands of forensic casework and databasing.
Overview:
The SeqStudio 8 Flex (HID edition) acts as a high-performance upgrade to legacy CE systems like the 3500 series. It features an 8-capillary configuration designed to balance daily sample processing needs with maximum workflow adaptability. A defining trait of the Flex series is its modern standalone footprint — featuring an integrated touchscreen computer and onboard data collection software, it operates independently without requiring an external desktop setup.
Key Features:
Continuous Plate Loading: A 4-plate capacity deck allows you to load new sample plates or 8-tube strips on the fly without interrupting or pausing the current run injection.
Sample Reprioritization: If an urgent case sample enters the lab, you can inject it immediately ahead of scheduled queues.
Auto-Spectral Calibration: Minimizes time-consuming "pull-up" data edits by utilizing sample-specific spectral data to automatically calibrate dye signals.
Redesigned Robust Array: Capillary arrays feature an enhanced click-and-slide retractor tab to protect the delicate detection window during handling and routine installations.
Consumable Backward Compatibility: It is fully compatible with the same polymers (POP-4™) and buffers used in the widely adopted Applied Biosystems 3500 Series Genetic Analyzers.
Smart Connectivity & Security: Integrates directly with Lab Information Management Systems (LIMS), local networks, and the cloud-based Thermo Fisher Connect Platform for remote run monitoring. It also includes an optional SAE (Security, Audit, and E-signature) module for strict data traceability.
Forensic Applications:
Short Tandem Repeat (STR) Analysis: Validated to work seamlessly with gold-standard Applied Biosystems STR kits (such as GlobalFiler™ and Yfiler™ Plus) as well as other standard 5-dye and 6-dye multiplex amplification chemistries.
Forensic Casework & Databasing: Optimized for processing reference samples, standard crime scene profiles, and complex or degraded DNA evidence.
Mitochondrial DNA Sequencing: Supports Sanger sequencing workflows used to analyze hypervariable regions of mtDNA in cases where nuclear DNA is absent.
Relationship & Paternity Testing: Accurate fragment sizing ensures dependable kinship analysis.
Technical Specifications:
Feature / Metric
Specification Details
Number of Capillaries
8 capillaries (Upgradable/interchangeable with 24-capillary arrays)
Dye Capacity
Up to 8-dye capable
Sample Format
4-plate deck; accommodates 96-well plates, 384-well plates, or 8-tube strips
Excitation Source
505 nm solid-state laser
Data Tracking
Radio Frequency Identification (RFID) for consumables; internal barcode reader
Throughput (HID Workflow)
298 samples per 23-hour window (using POP-4™ polymer & 36 cm array)
Run Time (per injection)
$\le$ 37 minutes
Sizing Precision
$< 0.15$ standard deviation for 60–460 base pairs
Secondary Analysis Software
Fully compatible with GeneMapper™ ID-X Software v1.7
Storage & Hard Drive
512 GB Solid-State Drive (SSD)
Dimensions & Weight
70 x 67.5 x 86.5 cm (W x D x H); 115 kg (253.5 lbs)
