Applied Biosystems SeqStudio 24 Flex Genetic Analyzer for HID
SeqStudio™ 24 Flex Genetic Analyzer
The Applied Biosystems™ SeqStudio™ 24 Flex Genetic Analyzer for Human Identification (HID) is a mid-to-high throughput capillary electrophoresis (CE) system. It is specifically optimized to meet the strict data accuracy, compliance, and efficiency demands of forensic laboratories and human identification workflows.
Overview:
The SeqStudio 24 Flex (HID configuration) functions as a true standalone system. It integrates a 24-capillary array with an onboard touchscreen computer and specialized Data Collection software, eliminating the absolute requirement for an external desktop computer. It is designed to maximize a lab's "walk-away" time while handling unpredictable, urgent forensic casework workloads.
Key Features:
Continuous Plate Loading: A 4-plate deck allows you to load new 96-well plates, 384-well plates, or 8-tube strips mid-run without interrupting or terminating ongoing injections.
Urgent Sample Reprioritization: Forensic labs often get high-priority cases. The software allows you to pause the queue, insert a critical sample, prioritize it next, and resume the normal queue seamlessly.
Automated Calibration & Reduced Pull-Up: Auto-spectral calibration utilizes sample-specific data to dramatically minimize "pull-up" or spectral bleed-through, drastically reducing the manual editing and data re-analysis time for complex STR profiles.
Smart Service Ecosystem: Built-in Smart Help and Remote Support features allow users to send log files and collaborate with technicians directly from the instrument touchscreen, resolving up to 70% of technical queries remotely.
RFID Consumable Tracking: Built-in Radio Frequency Identification (RFID) monitors reagent use, expiration dates, and lot numbers automatically for streamlined inventory management and clear chain-of-custody tracking.
Core Applications
The SeqStudio 24 Flex for HID is heavily validated and tailored for fragment analysis and genetic profiling:
Forensic STR Genotyping: Processing complex casework samples and reference samples using 5-, 6-, and 8-dye multiplex Short Tandem Repeat (STR) amplification kits.
Database Profiling: High-throughput processing of offender reference samples for uploading to national DNA databases (like CODIS).
Missing Persons & Kinship Testing: Running lineage markers (Y-STRs, X-STRs) and autosomal STRs to solve kinship and human identification cases.
Mitochondrial DNA Sequencing: Leveraging Sanger sequencing capabilities on the same machine to analyze highly degraded skeletal remains or hair shafts where nuclear DNA is unavailable.
Technical Specifications:
Parameter
Specification
Number of Capillaries
24
Capillary Lengths
36 cm or 50 cm
Dye Compatibility
Optimally supports up to 8-dye chemistry systems
Sample Capacity
4-plate deck (96-well, 384-well, or 8-tube strips)
Throughput (HID Workflow)
895 samples per 23-hour window (using HID_J6_36_POP4 module)
Sizing Precision
$< 0.15$ standard deviation for 60–460 base pairs
Polymer & Buffer Support
Fully compatible with standard POP-4™, POP-6™, and POP-7™ polymers
Excitation Source
505 nm solid-state laser
Secondary Analysis Software
Pre-validated for seamless data export into GeneMapper™ ID-X Software v1.7
Dimensions (W x D x H)
70 x 67.5 x 86.5 cm (27.6 x 26.6 x 34.1 in)
Weight
115 kg (253.5 lbs)
Connectivity
Wi-Fi dongle support, Local Area Network (LAN), LIMS compatibility, and Thermo Fisher Connect Cloud Platform
Compliance & Security
Optional Security, Audit, and E-signature (SAE) software module for regulatory compliance
