Sanger Sequencing

Capillary Electrophoresis Systems for Sanger Sequencing | Applied Biosystems

Sanger Sequencing

Overview: 

Today, DNA sequencing is a fundamental tool in basic, applied and clinical research.  Whether checking the integrity of a clone or the identity of a forensic sample, data quality is paramount to getting the right answer to your sequencing questions. Capillary electrophoresis is the gold standard for Sanger (DNA) sequencing and fragment analysis. Its utility is proven through decades of results and over 20,000 publications, including the first human genome and the discovery of genes implicated in diseases like cystic fibrosis. In addition to multiple Sanger sequencing–based applications, capillary electrophoresis is used in fragment sizing and quantification-based applications. Applied Biosystems® Genetic Analysis Systems continue to offer proven high-quality instrumentation, reagents, consumables, analysis software, and world-class technical support to respond to the unlimited potential of scientific discovery.

Features:

  • High accuracy sequencing with proven Sanger chemistry
  • Capillary electrophoresis technology for precise fragment separation
  • Flexible throughput options (1–96 samples depending on model)
  • Ready-to-use reagent kits and simplified workflow
  • Integrated data analysis software with intuitive interface
  • Compact design (SeqStudio) or scalable platforms (3500/3730)
  • Supports both sequencing and fragment analysis applications
  • Minimal hands-on time with automation capabilities
  • Compatibility with validated Thermo Fisher reagents and consumables

Specifications

  • Technology: Capillary Electrophoresis
  • Detection Method: Fluorescence-based detection
  • Number of Capillaries:
  • SeqStudio: 1–4 capillaries
  • 3500 Genetic Analyzer: 8 capillaries
  • 3500xL Genetic Analyzer: 24 capillaries
  • 3730xl DNA Analyzer: 96 capillaries
  • Read Length: Up to ~1000 bp (depending on chemistry and sample quality)
  • Run Time: ~30 minutes to 3 hours (application dependent)
  • Software: Integrated data collection and analysis software

Low to high sample throughput—select the genetic analyzer aligned to your laboratory’s scale, throughput demands, and application complexity:

 

Our Resources

Genetic Analyzer Brochure

SeqStudio Series

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