Human Leukocyte Antigen (HLA) Typing using Sanger

HLA Typing by Sanger Sequencing | GenDx Kits & Solutions | IGB

Human Leukocyte Antigen (HLA) Typing using Sanger

Overview:
IGB offers high-quality products for HLA typing based on Sanger sequencing, the long-standing gold standard in HLA genotyping.

These solutions support accurate, low-ambiguity typing of HLA class I and class II genes, with validated protocols and integrated software for seamless analysis.

Trusted by laboratories worldwide, GenDx Sanger-based HLA typing kits deliver robust and reproducible performance for both clinical and research applications.

Features:
    • Proven amplification strategy established since 2005
    • Extensive exon coverage for improved typing accuracy
    • Comprehensive set of group-specific sequencing primers

Specifications:
    • Excellent exon coverage reduces allele ambiguity
    • Additional exon sequencing enables resolution of ambiguous alleles
    • Genotype ambiguities resolved using Group-Specific Sequencing Primers (GSSPs)

    SBTexcellerator kits are available in two variants:

    • Core Kit:
        o Includes amplification and sequencing primers for essential exons

    • Full Kit (available only for DQA1 and DPA1):
        o Includes amplification and sequencing primers for essential exons
        o Additional sequencing primers for extended exon coverage
        o Multiple GSSPs for resolving genotypic and allelic ambiguities

Amplification Coverage:

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