Overview:
Reverse transcriptase (RT) enzymes are designed for high-efficiency conversion of RNA into complementary DNA (cDNA)—a critical step in workflows such as gene expression analysis, RT-PCR, RT-qPCR, RNA sequencing, and viral detection.
These reagents include standalone reverse transcriptases, optimized buffers, dNTPs, and complete cDNA synthesis kits, delivering high sensitivity, reproducibility, and full-length transcript coverage, even from low-abundance or partially degraded RNA samples.
Features:
1. Comprehensive Product Portfolio
• Reverse Transcriptase Enzymes:
o M-MLV based RT enzymes
o Engineered high-performance RTs (e.g., SuperScript™ series)
• cDNA Synthesis Kits:
o First-strand cDNA synthesis kits
o One-step RT-PCR kits
• Core Components:
o RT buffers
o dNTPs
o RNase inhibitors
o Primers (oligo dT, random hexamers, gene-specific)
2. Flexible Workflow Options
• Two-step RT-PCR: Separate cDNA synthesis and amplification
• One-step RT-PCR: Combined reverse transcription and PCR
• Compatible with:
o qPCR (SYBR/probe-based)
o NGS library preparation
