Human Identification Quantification and Validation
Quantifiler™ HP & Trio Kits | Forensic DNA Quantification | qPCR
Human Identification Quantification and Validation
Quantifiler™ HP & Trio DNA Quantification Kits
Supporting Systems: QuantStudio™ 5 Real-Time PCR System for HID
Features:
Dual-Target Quantification: Uses a multi-copy "Small Autosomal" (SA) and "Large Autosomal" (LA) target system to provide an accurate Degradation Index (DI).
Superior Sensitivity: Validated to detect DNA concentrations as low as <1 pg/µL, ensuring success even with minimal touch-DNA samples.
Male/Female Mixture Assessment: The Quantifiler™ Trio kit includes an improved Y-target to accurately quantify male DNA in the presence of high female DNA background (up to 1:4000 ratio).
Inhibitor Detection: Features an Internal PCR Control (IPC) to flag the presence of common forensic inhibitors (like hematin or humic acid) before proceeding to STR analysis.
Workflow Optimization: Results in approximately 1 hour, allowing labs to make "Go/No-Go" decisions for downstream STR kits (like GlobalFiler™ or VeriFiler™ Plus) immediately.
Specifications:
Feature
Quantifiler™ HP (Human Plus)
Quantifiler™ Trio
Primary Use
Total Human DNA quantification
Human + Male DNA quantification
Targets
Small Autosomal, Large Autosomal, IPC
Small Autosomal, Large Autosomal, Y-Target, IPC
Dynamic Range
0.005 ng/µL to >50 ng/µL
0.005 ng/µL to >50 ng/µL
Run Time
~60 Minutes
~60 Minutes
Validation Platform
QuantStudio™ 5 / 7500 Real-Time PCR
QuantStudio™ 5 / 7500 Real-Time PCR
Sensitivity
< 1 pg/µL
< 1 pg/µL
Validation Consumables & Services
Performance Verification & Calibration
ROI and Background Calibration Kits: Essential for maintaining the optical precision of the Real-Time PCR systems.
TaqMan™ RNase P Verification Plate: A specialized consumable used to verify the instrument's ability to distinguish between 2-fold quantity differences.
HID Verification Service: Professional on-site validation service (IQ/OQ/PQ) to ensure the entire system—from reagents to software—is ready for casework.
