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Human Identification NGS

Precision ID NGS System & Panels

Primary Platforms: Ion GeneStudio™ S5 System & Ion Chef™ System

Features:

Massive Multiplexing: Power of Ion AmpliSeq™ technology allows for the simultaneous amplification of hundreds of markers (STRs, SNPs, or mtDNA) in a single reaction tube.
Superior Sensitivity: Validated to produce high-quality, concordant profiles from as little as 125 pg of DNA.
Degraded Sample Recovery: Small amplicon designs (often <140 bp) specifically engineered for highly compromised samples like skeletal remains, rootless hair, and old stains.
Automated Workflow: Hands-free library preparation and chip loading via the Ion Chef™ System, reducing manual pipetting errors and bench time.
Deep Investigative Insights: Beyond simple STR matching, NGS provides biogeographic ancestry, phenotypic traits (eye/hair color), and sequence-level STR variation (isometric alleles).

Specifications:

Feature	Precision ID GlobalFiler™ NGS STR Panel	Precision ID Identity & Ancestry Panels	Precision ID mtDNA Whole Genome Panel
Marker Type	35 markers (21 CODIS STRs + sex + 14 extra)	124 Identity SNPs / 165 Ancestry SNPs	Full Mitochondrial Genome (16.5 kb)
Input DNA	≥125 pg	≥125 pg	≥100 pg
Lysis to Data	< 2 Days	< 2 Days	< 2 Days
Hands-on Time	< 45 minutes (with Ion Chef)	< 45 minutes (with Ion Chef)	< 45 minutes (with Ion Chef)
Sequencing Time	~2–4 hours	~2–4 hours	~2–4 hours
Data Analysis	Converge™ Software	Converge™ Software	Converge™ Software

Category	Panel	Ion S5 chips Number of Smples per run
		Ion 510 Chip	Ion 520 Chip	Ion 530 Chip
mtDNA	Precision ID mtDNA Whole Genome Panel		25	32
mtDNA	Precision ID mtDNA Control Region Panel	37	56
STR	Precision ID GlobalFiler NGS STR Panels		16	32
SNP	Precision ID Ancestry Panel	48	72	362
SNP	Precision ID Identity Panel	54	81	384

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Human Identification NGS

Precision ID NGS System & Panels

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