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Oncomine Myeloid Research Assay

One single assay for comprehensive coverage of relevant myeloid mutations

Current methods for the analysis of hematological malignancies often involve multiple sequential tests and laborious workflows. Adoption of next-generation sequencing (NGS) into clinical research laboratories has created an unprecedented opportunity to profile the multiple relevant driver genes in myeloid malignancies in a single test.

The Ion Torrent Oncomine Myeloid Research Assay is a comprehensive, targeted NGS assay designed to assist in the understanding of myeloid cancer. Specifically, it interrogates relevant DNA mutations and fusion transcripts associated with myeloid disorders in a quick and easy NGS run. Our panel is comprised of 40 key DNA target genes and 29 driver genes in a broad fusion panel (Table 1) to cover all the major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).


  • Ease of use—one simple, robust workflow with all mutations encapsulated in two DNA and one RNA pool
  • Effective designs—great coverage of challenging targets such as CEBPA and internal tandem duplications of FLT3 (FLT3-ITDs)
  • Flexibility—verified with blood and bone marrow samples on the Ion PGM and Ion GeneStudio S5 systems, with manual and automated library preparation
  • Speed—from samples to answers in <3 days, with up to four samples on an Ion 318 Chip or twelve on an Ion 530 Chip
Oncomine Myeloid Research Assay

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