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LGC SeraCare – Liquid Biopsy Assays

What are the VAF ranges for the ctDNA products?

  • Seraseq Myeloid ctDNA Mix: 0% (WT), 0.1%, 0.5%, 1.0%
  • Seraseq ctDNA v2 Reference Material: 0% (WT), 0.125%. 0.5%, 1%, 2%
  • Seraseq ctDNA v2 Mutation Mix: 0% (WT), 0.125%, 0.25%, 0.5%, 1%, 2%
  • Seraseq Complete Reference Material: 0% (WT), 0.1%, 0.5%, 1%, 2.5%, 5%
  • Seraseq Complete Mutation Mix: 0% (WT), 0.1%, 0.5%, 1%, 2.5%, 5%

In what formats are the products available for sale?

  1. Purified ctDNA “Mutation Mix” – Directly used for NGS Library Preparation
  2.  Encapsulated format (plasma-like material) “Reference Material”

How are these VAFs determined?

Measured allele frequencies can be found in the individual Technical Product Reports or CoAs.

What NGS chemistry is recommended to analyze these reference samples?

The Seraseq ctDNA reference materials are designed for targeted NGS assays based on amplicon or hybrid-capture chemistries. These should be compatible with most common NGS assays from Illumina, ThermoFisher, Archer, Qiagen or Roche Dx.

For the encapsulated format, does SeraCare recommend a cfDNA extraction kit?

No, we do not officially recommend an extraction kit for use with the Seraseq ctDNA reference materials.

What is the typical yield of ctDNA recovered from a single vial of the RM product?

Using the cfDNA extraction kit from Qiagen and Qubit for quantitation, typical ctDNA yields from a 1 mL volume of encapsulated material range between ~25-35 ng/mL This is based on triplicate extractions.

What is the typical ctDNA fragment size in the Seraseq ctDNA products?

Typical range is 155-185 bp across all VAFs and WT samples.

Is the background free of mutations?

Our ctDNA materials are generated using GM24385 cells. The propagation and production of our materials maintains a background that is generally consistent with the published genome sequence of GM24385.

How do I use the MRD ctDNA Panel?

The MRD ctDNA Panel mix is designed to support development and validation of ctDNA-based patient-informed minimal residual disease (MRD) assays, mainly for solid tumors.

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