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The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive, and broad-range research analyses of mixed microbial populations using the Ion PGM™ System. The kit uses two primer pools to amplify seven hypervariable regions (V2, V3, V4, V6, V7, V8, and V9) of bacterial 16S rRNA. The combination of the two primer pools enables broad-range, sequence-based identification of bacteria from complex mixed populations.
Ion 16S Metagenomics Kit
The amplified fragments can then be sequenced on the Ion PGM™ System and analyzed using the metagenomics workflow in the Ion Reporter™ Software.
Integrated, cost-effective, scalable 16S rRNA sequencing workflow for the Ion Torrent sequencing systems, with a rapid workflow from the library to analyze results. Following library construction and template preparation, 400 bp sequencing runs are completed in as little as 4 hours. BAM files produced with Torrent Suite™ Software are automatically analyzed, annotated, and taxonomically assigned in the Ion Reporter Software 16S metagenomics workflow.
Benefits of the Ion 16S ™ Metagenomics Kit include:
- Use starting material from direct samples without culture, mixed culture, or pure culture
- Primer sets for multiple V regions allow accurate detection and identification of a broad range of bacteria down to genus or species level
- Flexibility—primer sets provided as two pools allow for separate analyses using individual pools
- Primers can also be barcoded
16S Sequencing Application Notes
Ion Torrent™ sequencing is cited in more than 900 peer-reviewed publications on microbial sequencing for bacterial and viral typing research, metagenomics, and the discovery of unknown microorganisms.
Integrated, cost-effective, scalable 16S rRNA sequencing workflow for the Ion GeneStudio S5 System, with a rapid workflow from the library to analyzed results. Following library construction and template preparation, 400 bp sequencing runs are completed in just 4 hours for the Ion 520™ and Ion 530™ Chips. BAM files produced with Torrent Suite™ Software are automatically analyzed, annotated, and taxonomically assigned in the Ion Reporter Software 16S metagenomics workflow.
