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Genomed Aneufast QF-PCR

The Aneufast™ Kit is a molecular diagnostic assay designed for rapid detection of chromosomes 13, 18, 21, X and Y aneuploidies by Quantitative Fluorescent PCR (QF-PCR). The Aneufast™ QF-PCR Kit contains a total of 36 markers in six multiplex reactions that amplify selected Short Tandem Repeats (STRs) and the gender determining sequences Amelogenin-SRY. Two multiplex QF-PCR amplifications of 21 markers (S1 and S2) are designed to be analysed in a single electrophoresis to increase sample throughput. The inclusion of 4 chromosome specific extra markers sets allows definitive results in 100% of cases.

Rapid Prenatal Diagnosis: Aneufast™ QF-PCR Kit Workflow

Aneuploidy screening is performed by amplifying five STRs on chromosomes 13, 18, 21, three pseudoautosomal markers, one X linked, as well as the AMXY and SRY. Markers are distributed in two multiplex QF-PCR assays (S1 and S2) of the Aneufast™ Kit, in order to reduce the risk of sample mishandling. Following collection of the products and simultaneous electrophoretic analysis, agreement between results from the two multiplexes allows diagnosis to be performed with two independent assays on each sample.

Samples with fewer than two informative markers on each chromosome can be tested using chromosome-specific multiplex PCR assays to include up to eight STRs on chromosomes 21 and 18, seven on chromosome 13, eight markers on the X and four on both XY. These sets of additional markers (M13, M18, M21 and MXY), which are included in the Aneufast™ QF-PCR kit, can also be useful for additional confirmation of sample identity in all aneuploidy cases, by testing a second aliquot obtained from the original sample.

Features

  • Sample to result in less than 3 hours
  • Extra Markers on Chromosomes 13, 18, 21, X and Y included in the box
  • Optimised to work on Sanger sequencers
  • Five Dye-Labeling allows simultaneous analysis of several loci
  • Only product on the market with 4 pseudoautosomal markers for XY aneuploidy and mosaic cases

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