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EVOS Onstage Incubator

The second-generation EVOS Onstage Incubator (OSI-2) is an accessory for EVOS M5000 and EVOS M7000 Imaging Systems that enables the incubation of cells at user-defined temperature, humidity, and gas (O2, CO2 or N2), for capture of images and recording of time-lapse movies of live-cells under physiological and non-physiological conditions (e.g., hypoxia) over long periods of time.

Key Features

  • Intuitive user-interface (UI) simplifies experiment set-up and monitoring of experimental conditions
  • UI enables adjustments while experiments are running
  • Small footprint and sleek design saves lab space
  • Onboard air compressor eliminates need for separate air-tank; OSI-2 is also quiet at 42 dB
  • Precise control over temperature (see figure below), humidity, and gasses provides relatively uniform live-cell environment whether physiological or non-physiological conditions (e.g., hypoxia)

Onstage incubator vessels and vessel holders

To ensure proper operation of the EVOS Onstage Incubator, we recommend choosing vessels designed specifically for live-cell imaging. Conventional plastic plates can generate optical problems or even autofluorescence. Additionally, it is important to use only EVOS Onstage incubator compatible vessel holders and stage plates.

Onstage incubator media

Many cell culture media are carbonate buffered and, once removed from the incubator, the relatively lower CO2Ā concentration of air will cause an increase in the medium pH within an hour. The optically clear, physiological solution (Live Cell Imaging Solution, or LCIS) maintains physiological pH and osmolarity for up to 4 hours at ambient atmosphere and temperature. This is especially useful for imaging outside of an incubator but can also help to maintain pH while using the EVOS Onstage Incubator-2.

For longer-term imaging, particularly in a microscope-stage incubator, we offer a clear cell culture medium,Ā Gibco FluoroBrite DMEM, that is based on DMEM to help preserve cell health while also providing low background fluorescence (comparable to that of phosphate-buffered saline and 90% lower than standard phenol redā€“free DMEM).

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