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Nucleic Acid (NA) Isolation, Quantification & Quality assessment

Nucleic Acid (NA) isolation is a key step in many molecular biology workflows impacting downstream steps especially enzymatic reactions. Commonly used biological samples used for life sciences are as tabulated below:

Clinical and HLA Applied Markets Basic Research
Blood, tissue biopsies, Circulating tumor cells (CTC’s), Stem cells, Cultured cells, Urine, feces, buccal swabs, sputum, hair follicles,
Formalin Fixed Paraffin embedded (FFPE) samples
Cultured cells, environmental samples-soil, bio-waste, food samples, forensic samples, animal tissues Tissues-Plants or animals, culture cells, Single cell analysis, blood, bacterial cultures

Solutions from

The challenges posed by each of the samples w.r.t NA isolation is unique. While environmental samples require steps to remove inhibitors, clinical samples such as urine require additional concentration steps to collect NA in small volumes and forensic crime scene samples could be limiting in quantity and subject to harsh environments degrading the nucleic acid in the sample.

  • DNA
  • RNA
  • Common Equipment

Sample collection

Sample collection incorporating good laboratory practices include:

  • Use of nuclease free tubes, tips, buffers and water
  • Cleaning surfaces using DNA away to eliminate DNA exonuclease and endonuclease contamination
  • Use of appropriate gloves.
  • Protection of surfaces using bench protectors.

Clinical samples and those suspected to contain infectious material should be handled with the relevant Biosafety precautions- biosafety cabinets, hoods, appropriate clothing & gloves and other handling guidelines.

More biosafety solutions.

For Clinical solutions, non-invasive samples such as Saliva can be collected using kits such as Oragene•DNA or ORAcollect•DNA can be deployed. These contain stabilization agents for storage and shipment of saliva samples for testing.

Oragene•DNA (OG-510)
Oragene•DNA (OG-575) for Assisted Collection
Oragene•ONE (ON-500)
ORAcollect•DNA (OCR-100)

For storage of buffy coat samples, HEMAgene®•BUFFY COAT (HG-BCD) allows stabilization of DNA and transportation at ambient temperature, ease of use with multiple workflows.


Homogenization

Adequate disruption of the cellular or tissue architecture to release the nucleic acids in the cell can affect the quality and quantity of Nucleic acid isolated. Hence, the homogenization step in the protocol has to be optimized based on sample type and source. Several tools and options are available to achieve a homogeneous cell mixture suitable for downstream Nucleic acid (NA) isolation. The following table captures most often deployed solutions:

Sample type Sample properties Homogenization & other solutions Procedure Modifications
Animal Tissues Hard tissues (bones, teeth), nuclease-rich (spleen, liver), fatty tissues (brain) TH Tissue Homogenizer
Tissue Master
THQ Digital Tissue homogenizer
Hard Tissue OMNI Tip
Soft Tissue OMNI Tip
Omni General Laboratory Homogenizer (GLH 850)
Thorough disruption with appropriate tissue homogenizer and tips and ensure complete mixing with lysis buffer from DNA/RNA isolation kits
Alternate Freeze tissue and grinding
Food & Plant Hard grains, emulsions, large volumes,
Fibrous and difficult tissues to disrupt
Omni Prep homogenizer with Omni Hard Tissue Tips
Omni Mixer
Omni General Laboratory Homogenizer (GLH 850)
Optimize homogenization, collect debris by centrifugation and re-homogenize
Environmental Bacterial sample cell wall lysis critical to release nucleic acids
Nanoparticle dispersion
Omni Ruptor 4000 Ultrasonic Homogenizer
Omni Sonic Ruptor 400 Homogenizer
Bead Ruptor
Micro-Organism Lysing Mix (2 mL Tubes) Nuclease Free- 50 Pack
Bead Mill Bacterial DNA Purification Kit – 50 Prep
Ensure thorough disruption, for bacteria- centrifuge and re-sonicate or homogenize bacterial pellet.
Soil Samples Bead Ruptor
Micro-Organism Lysing Mix (2 mL Tubes) Nuclease Free- 50 Pack
Soil DNA isolation kit
Cellular From blood, cell cultures, phage particles Bead Ruptor
Omni Mixer
Omni Ruptor 4000 Ultrasonic Homogenizer
Ensure complete mixing with lysis solution after homogenization

DNA isolation

DNA isolation or genomic DNA (gDNA) isolation protocols aims to dissociate DNA from the cellular protein machinery and release into solution. Most commonly used solutions for DNA isolation employ chaotropic salts with or without organic components (phenol) to lyse the cell. This step prevents DNA degradation by exo or endonucleases in the cell. Subsequently, some kind of separation- either on a column, by centrifugation or magnetic bead based methodology is used to capture the pure DNA.

Special kits for DNA isolation from tissues, plants, forensic and microbial samples are:

Tissues, Cells & Blood
Plants
Forensic samples
Food & Microbial samples

Automation using KingFisher instrument and solutions is available for processing 24 to 96 samples or higher in a single run.

Download brochure

Quick TipsPlant RNA isolation aid

Automation solutions for DNA isolation

MagMAX™ Express Magnetic Particle Processor
MagMAX™ Express-96 Deep Well Magnetic Particle Processor
MagMAX™ Express-96 Standard Magnetic Particle Processor
Learn more about MagMAX instruments

Genomic DNA from Tissues, Cells and Blood

DNAzol Reagent for whole blood DNAzol™ Reagent for solid and liquid samples PureLink ™ Genomic DNA Mini Kit MagMAX™ DNA Multi-Sample Ultra Kit
Catalog Number 10974020 10503027 K182001 A25920
Isolation technology Uses novel organic & guanidine-detergent lysing solution selectively allows DNA precipitation while lysing RNA. (Manual- not for high throughput) Uses novel organic & guanidine-detergent lysing solution selectively allows DNA precipitation from cell lysate. (Manual- Not for high throughput) Silica spin column methodology
(Manual- not for high throughput)
Magnetic bead–based purification scalable from processing 12 to 500 samples a day. Automated Protocols, High Throughput-Compatible, Manual Protocols
Prep time 30 minutes 10-30 minutes 20-30 minutes ~45 minutes
Sample Types Whole blood, serum samples for viral DNA Cell, whole blood or tissues Blood, Tissues, cells, Bacteria, swabs, and blood spots, with a familiar Whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue
Starting Material 1 ml of DNAzol® Reagent for genomic DNA from 0.5 ml of whole blood to yield 10-20 µg DNA. Can be used for isolation of apoptotic fragments from whole blood and viral DNA from serum 1 mL of DNAzol® Reagent can be used to isolate genomic DNA from 1–3 x 107 cells, from 0.1 mL of whole blood, or from 25–50 mg tissue. 5 x 10^6 to 1 x 10^8 cells, Up to 200 mg tissue 1 buccal swab
Whole blood- 50 to 350 µL
Urine (not conc)- up to 400 µL 
Saliva up to 150 µL
Mouth Rinse ( 1-5 mL )
Blood spot cards
Mammalian tissue (up to 10 mg)
Applications Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization. Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization. Genotyping, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)

Genomic DNA from Plants

ChargeSwitch™ gDNA Plant Kit MagMAX™ Plant DNA Isolation Kit Plant DNAzol Reagent
Catalog Number CS1800010 A32549-96 preps
A32580-384 preps
10978021
Isolation technology Magnetic Bead based extraction.
Pure DNA with no guanidine or organic contamination.
High Throughput-Compatible
Magnetic bead technology, eliminates the need for phenol/chloroform extraction & alcohol precipitation.
High throughput-Compatible
Novel organic & guanidine-detergent lysing solution for selective DNA precipitation and RNA lysis. Not High Throughput-Compatible (Manual)
Prep time 15 minutes 30 minutes 60-70 minutes
Sample Types Fungal and plant samples, including leaves, tissues and seeds Suitable for most plant types and parts, including woody, polyphenol-rich, and lignified samples Plant tissues such as leaf, seed stem, root, and callus
Starting Material 50-100 mg of fungi and plant samples- including leaves, tissues & seeds to yield up to 7 µg of gDNA 10–100 mg plant samples Up to 100 mg tissue, Up to 5×106 cells
Applications Genetically modified organism (GMO) screening, PCR, restriction enzyme digestion, and Southern blotting. PCR, Real-Time PCR (qPCR)
Next-generation sequencing (NGS)
Cloning, PCR, Sequencing, Southern Blotting

Genomic DNA from Forensic samples

AutoMate Express™ Forensic DNA Extraction System MagMAX™-96 DNA Multi-Sample Kit ChargeSwitch® Forensic DNA Purification Kit iPrep™ ChargeSwitch™ Forensic Kit
Catalog Number 4441763 4413022 CS1120010 IS10002
Isolation technology Bench-Top instrument which utilizes PrepFiler Express™ and PrepFiler Express BTA™ chemistries packaged in pre-filled, foil sealed cartridges. Protocol card pre-programmed for purifying nucleic acids from forensic samples. Magnetic bead-based nucleic acid isolation technology free from inhibitors which affect downstream reactions: genotyping assays, CE and next-generation sequencing, and high resolution melting analysis Magnetic bead-based technology to isolate genomic DNA without the need for hazardous chemicals, centrifugation, or vacuum manifolds
(Ideal for automation and high throughput applications)
ChargeSwitch® technology is based on an unique, ionizable nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification avoids the use of guanidine, ethanol, and other reagents.
Not High Throughput-Compatible (Manual)
Prep time 30 minutes (up to 13 samples) 20 minutes 20-30 minutes 20 minutes
Sample Types Challenging samples such as bones, teeth and adhesive based samples such as cigarette butts and tape lifts
  • Blood and blood cards, tissue
  • buccal swabs
  • Buffy coats, and cultured cells. Ideal for samples containing enzymatic inhibitors matrix (Haeme, Heparin, salts, protein) and removal of inhibitors from the purification method itself (phenol, salts, alcohol)
Suited for environmentally degraded samples

  • Dried blood spots on paper, clothing
  • Hair follicles and hair shafts
  • Cigarette butts
  • Cigarettepaper
  • Envelopes
  • Drinking vessel swabs
  • Chewing gum
  • Sperm head cells
  • Vaginal epithelial cells
  • Door handle swabs
  • Strip removed cells (e.g. hats, coats, and gloves)
  • “Touch DNA” (e.g. tools, mobile phones, and microscopes)
  • Dyed denim
  •  Dried blood spots on paper
  • and clothing
  • Hair follicles and hair shafts ·
  • Cigarette butts
  • Semen
  •  “Touch DNA” (e.g. tools, mobile phones, and microscopes)
Applications For Research, Forensic or paternity genotyping tests analyzing short tandem repeats (STRs) Genotyping, PCR, Real-Time Quantitative PCR (qPCR), Sequencing Forensic Crime scene sample types for STR profiling, PCR Cloning, Forensic Testing, PCR, Sequencing, Southern Blotting

Genomic DNA for Food Safety and Microbial Testing applications

PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing PureLink™ Pro Quick96 Plasmid Purification Kit
* non-genomic (plasmid DNA) prep
MagMAX™ Total Nucleic Acid Isolation Kit
Catalog Number 4480466 K211024A AM1840
Isolation technology Enzymatic & magnetic beads. Automated Protocols, High Throughput-Compatible. Ideal with MagMAX™ Express-96 Deep Well Magnetic Particle Processor Silica plate extraction chemistry with 96-well plate design for manual or automated processing of plasmid DNA.
Automated Protocols, High Throughput-Compatible
Zirconia beads for the mechanical disruption of difficult pathogens. DNA isolation using magnetic bead technology.
Prep time 6-20 hrs (based on Microbial enrichment step) <45 minutes ~45 minutes
Sample Types Bacteria, Food & Environmental Samples, Liquid Samples (e.g. Serum) E. coli strains grown in up to 5 ml LB, 3 ml 2xYT, or 3 ml TB Bacterial and cell culture samples
Starting Material 100 mg of tissue or 107 cells (requires 1 mL reagent) 1.2-1.5 ml lysate⁄well yielding up to 15ug plasmid DNA 175 µl liquid samples of bacterial cultures
Applications PCR, Real-Time Quantitative PCR (qPCR), Ideal for Listeria, E.coli, Salmonella and other bacteria testing in Food and environmental samples Cloning, Next-Generation Sequencing, PCR, Sequencing, Restriction Enzyme digestion. Veterinary use for PCR, Real-Time PCR (qPCR)

Other kits: PureLink™ Microbiome DNA Purification Kit (Invitrogen™)
Vacuum manifold for plasmid purifications: FastVac™ Vacuum Manifold


DNA quantitation & quality assessment

Assessment of nucleic acid concentration can be done via spectrophotometric or fluorescence based measurements. Spectrophotometric absorbance measurements at 260 nm and 280 nm, provide an estimate of the amount of the DNA or RNA and protein in the sample. Alternate measurements at 230 nm allow evaluation of contaminants from the NA isolation protocol such as phenol. Various applications require different concentrations of sample and purity. Ratios of A260/A280 nm of 1.7 or higher indicate high quality nucleic acid ideal for most applications.

Fluorescence measurements with dyes that specifically bind dsDNA or RNA are recommended for applications such as Next Generation Sequencing, Microarrays and high throughput Real-time PCR (qPCR). Specialized fluorimeters such as the Qubit 2.0 or 3.0 systems with dedicated kits allow NA measurements for such applications. For more on comparison between Absorbance vs. Fluorescence see below and read more at:

Absorbance Measurements
SpectraMax QuickDrop Micro-Volume Spectrophotometer
Fluorescence Measurements

UV measurement guidelines for RNA analysis

Absorbance Indicates presence of Target ratios
230 nm Organic compounds, sugars, urea, salts A260/A230 > 1.8
260 nm All nucleic acids A260 ≈ 0.1–1.0
270 nm Phenol A260/ A 270 > 1.0
280 nm Protein RNA: A260/A280 ≈ 2.0
DNA: A260/A280 ≈ 1.8
330 nm Light scattering A330 = 0
Absorbance Measurements Fluorescence Measurements
Cannot discriminate between DNA and RNA if both present in the sample Quantitation done by binding of specific dyes unique for dsDNA, RNA, small RNA- measures exact level of each species
Not ideal for very limited sample concentrations 10ng/µL and lower Ideal for very limited sample concentrations 1 ng/µL or lower
Kits Sample starting concentration range for kit
Qubit ds DNA HS assay 10pg/µL-100ng/µL
Qubit sdDNA BR assay 100pg/µL-1µg/µL
Qubit ssDNA assay 50pg/µL-200 ng/µL
Qubit RNA assay 250 pg/µL-100ng/µL
Qubit RNA BR assay 1ng/µL-1µg/µL
Qubit protein assay 12.5µg/mL-5mg/µL
Quick TipsEnsure optimal RNase I treatment of DNA samples. To check, run on an 1% agarose with appropriate size standards to visualize high molecular weight DNA and ensure RNA (smear at low molecular weights) is removed

DNA storage


High mol. weight genomic DNA

Storage of purified DNA after isolation can be carried out based on long term or short term usage. Ideally, DNA should be stored in buffered solutions such as Tris EDTA (TE) at pH 8.0. A small aliquot of DNA can be stored at 4°C refrigerators for repeated uses. For long term storage, aliquot and freeze in -80°C freezers. Frozen samples should not undergo repeated freezing and thawing as this degrades large molecular weight genomic DNA.


Typical steps in a manual RNA isolation

Sample collection, stabilization and storage

Working with RNA poses its own set of challenges. RNA is required for studies involving Gene Expression, Transcriptome analysis, small RNA and microRNA analysis (miRNA), RNA interference (RNAi) , structural RNA studies or RNA virus detection.

Unlike its deoxy counterpart, the ribonucleic sugar in RNA is susceptible to alkaline hydrolysis. Once released out of the cell, the RNA is prone to degradation by Ribonucleases (RNAses) which are ubiquitous in the sample and surroundings.

Quick TipsIGB Tips: 4 R’s for good quality RNA!

RNAasefree sample collection using Nuclease free tips, tubes, buffers & decontaminate surfaces with RNAzap
Resuspend samples  immediately in RNAlater™ Stabilization Solution
Research ideal combination of isolation protocols: Combinations suited for all sample in the experiment (Guide )
Recovered RNA should be stored in The RNA storage or RNAsecure™ Resuspension Solution

If the experiment involves work with infectious agents additional protection will need to be deployed as described below:
Protection of surfaces using bench protectors. Clinical samples and those suspected to contain infectious material should be handled with the relevant Biosafety precautions- biosafety cabinets, hoods, appropriate clothing & gloves and other handling guidelines. Visit for more biosafety solutions.
The Oragene•RNA for Expression Analysis Self-Collection Kit is the only all-in-one system for the collection, stabilization and transportation of high quality mRNA from saliva.


RNA isolation

Protocol modifications to improve RNA yield and quality

Tissue Characteristics Symptoms Representative Tissues Procedure Modifications
Fibrous Tissues
(put pictures here)
Difficult to homogenize
  • Heart muscle
  • Skeletal muscle
Thorough disruption with appropriate tissue homogenizer/ Freeze tissue and grind
Nucleic Acid-rich Viscous lysate, incomplete phase separation, precipitation on addition of isopropanol
  • Spleen
  • Thymus
Dilute lysate 
Do additional extraction
Protein and Lipid-rich White flocculent material in aqueous phase of extraction
  • Brain
  • Plant Tissue
Dilute lysate 
Modify extraction(more CHCI3)
Nuclease-rich Degraded RNA
  • Spleen
  • Pancreas
  • Thymus
Efficient disruption 
Freeze tissues and grind
Quick TipsGuide & Brochure: Match our RNA isolation solution to your experimental needs!

RNA isolation from tissues, cells and blood

TRIzol Reagents Trizol Plus Reagents PureLink Mini Kits MagMAX Kits
Catalog Number 15596026 12183555 12183020
(other larger size kits available)
AM1840
Isolation technology Organic phenol extraction and alcohol precipitation. Manual- Not for high throughput Dual purification using Organic extraction and spin columns. (Manual- Not for high throughput) Silica spin column based methodology
(Manual- not for high throughput)
Scalable, flexible format with magnetic beads (Ideal for high throughput applications)
Prep time ~1 hour 20 minutes < 20 minutes ~45 minutes
Sample Types Bacteria, Blood, Cells, Plant Samples, Tissue, Viral Samples, yeast. Ideally suited for samples that are difficult to lyse Bacteria, Cells, Plant Samples, Tissue, Yeast
*Highly recommended for fatty and nuclease rich samples
Bacteria, Blood, Cells, Plant Samples, Tissue, Yeast,
*specific formulation kits available for blood, virus, miRNA
Kits for cell-free, Tissues, cells, stabilized blood, serum/plasma, urine, plants, viral, pathogen extraction and FFPE samples
Starting Material 100 mg of tissue or 107 cells (requires 1 mL reagent) Up to 200 mg tissue, Up to 5 x 108 cells 5 x 10^6 to 1 x 10^8 cells, Up to 200 mg tissue Up to 100 mg tissue, Up to 5×106 cells
Applications Cloning, Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction Microarray Analysis, Northern Blotting, Nuclease Protection Assays, Nucleic Acid Labeling, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction Microarray Analysis, Next-Generation Sequencing, Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction Northern Blotting, PCR, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting
Types of RNA isolated Total RNA, Transcriptome RNA, micro RNA Total RNA
(yield up to 1000ug)
Total RNA Total RNA, microRNA

Microbial RNA or RNA & DNA isolation solutions

RiboPure™ RNA Purification Kit, bacteria MICROBEnrich
Kit
MagMAX™ Pathogen RNA/DNA Kit PureLink® Viral RNA/DNA Mini Kit
Catalog Number AM1925 AM1901 4462359 12280050
Isolation technology Dual organic and Silica column based method for isolation of RNA. Manual (not for high throughput applications) Magnetic bead based hybridization capture. Enriches for microbial mRNA in a host-microbe mixed RNA sample by selective depletion of host mRNA and rRNA.
Host: Human, mouse, rat
Isolation of RNA and DNA from viruses and G- bacteria. Kit based on magnetic particle based 96-well format.
Hi-throughput instruments:
KingFisher™ Flex Magnetic Particle Processor, MagMAX™ Express Magnetic Particle Processor, MagMAX™ Express-96 Deep Well Magnetic Particle Processor, MagMAX™ Express-96 Standard Magnetic Particle Processor
The kit is designed for isolation of viral RNA or DNA in a low volume. It is a silica spin column based isolation resulting in NA free of proteins and nucleases. The final NA is eluted in 10-50uL elution volumes typically used for viral detection and genotyping.
Prep time About 45 mins 45 minutes <45 minutes
Sample Types Ideal for difficult to lyse G+ bacterial samples Host-microbe mix total RNA Serum and plasma; nasal, tracheal, and cloacal swabs; ear notches)
• Whole blood
• Semen
• Oral fluid
• Feces
• Biomed Diagnostics InPouch™ TF (Tritrichomonas foetus) culture
Cell-Free Fluids, Cerebrospinal Fluid, Plasma, Serum
Starting Material Up to 10^9 cells 20 reactions x 25 µg RNA total RNA per reaction Variable Viral RNA and DNA from ≤500 µL cell-free samples
Applications RNA sequencing, microarray analysis, real-time PCR (qPCR), reverse transcription qPCR (RT-qPCR), northern blotting, and cDNA library construction Microarray analysis PCR, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) Northern Blotting, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Type of RNA isolated Total RNA Total RNA Total RNA Total RNA

Automation solutions for RNA isolation

MagMAX™ Express Magnetic Particle Processor
MagMAX™ Express-96 Deep Well Magnetic Particle Processor
MagMAX™ Express-96 Standard Magnetic Particle Processor
Learn more about MagMAX instruments


RNA quality and quantification

Assessment of nucleic acid concentration can be done via spectrophotometric or fluorescence based measurements. Spectrophotometric absorbance measurements at 260 nm and 280 nm, provide an estimate of the amount of the DNA or RNA and protein in the sample. Alternate measurements at 230 nm allow evaluation of contaminants from the NA isolation protocol such as phenol. Various applications require different concentrations of sample and purity. Ratios of A260/A280 nm of 1.7 or higher indicate high quality nucleic acid ideal for most applications.

Fluorescence measurements with dyes that specifically bind dsDNA or RNA are recommended for applications such as Next Generation Sequencing, Microarrays and high throughput Real-time PCR (qPCR). Specialized fluorimeters such as the Qubit 2.0 or 3.0 systems with dedicated kits allow NA measurements for such applications. For more on comparison between Absorbance vs. Fluorescence see below and read more at:

Absorbance Measurements
SpectraMax QuickDrop Micro-Volume Spectrophotometer
Fluorescence Measurements

UV measurement guidelines for RNA analysis

Absorbance Indicates presence of: Target ratios
230 nm Organic compounds, sugars, urea, salts A260/A230 > 1.8
260 nm All nucleic acids A260 ≈ 0.1–1.0
270 nm Phenol A260/ A 270 > 1.0
280 nm Protein RNA: A260/A280 ≈ 2.0
DNA: A260/A280 ≈ 1.8
330 nm Light scattering A330 = 0
Absorbance Measurements Fluorescence Measurements
Cannot discriminate between DNA and RNA if both present in the sample Quantitation done by binding of specific dyes unique for dsDNA, RNA, small RNA- measures exact level of each species
Not ideal for very limited sample concentrations 10ng/µL and lower Ideal for very limited sample concentrations 1 ng/µL or lower
Kits Sample starting concentration range for kit
Qubit ds DNA HS assay 10pg/µL-100ng/µL
Qubit sdDNA BR assay 100pg/µL-1µg/µL
Qubit ssDNA assay 50pg/µL-200 ng/µL
Qubit RNA assay 250 pg/µL-100ng/µL
Qubit RNA BR assay 1ng/µL-1µg/µL
Qubit protein assay 12.5µg/mL-5mg/µL
Quick TipsEnsure optimal DNase I treatment of RNA samples. To check run on 1% agarose with appropriate size standards to  ensure no high molecular weight bands corresponding to gDNA.  Additional QC checks with  Qubit assays can help measure concentration of RNA in the final isolate.

RNA storage

Purified RNA should be stored in THE RNA storage or RNA secure™ Resuspension Solution.
Ideally, RNA should be stored in at neutral or slightly acidic conditions. A small aliquot of RNA can be stored at 4°C Refrigerators for repeated uses. For long term storage, aliquot and freeze in -80°C Freezers. Frozen samples should not undergo repeated freezing and thawing as RNA is very susceptible to degradation.


RNA & DNA isolation resources for various applications

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