Next Generation Sequencing (NGS)

Home / Applications / Techniques & Featured Technique / Next Generation Sequencing (NGS)

Next Generation sequencing (NGS) is a high throughput (DNA and RNA) sequencing methodology which has made immense impact on Cancer research, Reproductive genomics, HLA typing, Metagenomics, Human identification in Forensics, Infectious disease identification and several other areas. Common applications that have been implemented on NGS platforms include Gene Expression studies, Chromosome counting, Genotyping and STR analysis.

Solutions from

Our solutions for NGS are based on Thermo Scientific Ion Torrent chemistries which record a change in pH after each base (dNTP) addition to the growing DNA polymer. These measurements use a semiconductor based technology allowing measurement of millions of changes in a simple workflow. This method with targeted sequencing approach introduces a PCR-based sequence enrichment step using Ion AmpliSeq technology that focuses on genes or even genetic variants of interest; a good example is the targeting of oncogenes and tumor suppressors in a cancer research study.

Ion Torrent Applications

Solutions for NGS Sequencing Workflow

Sample Prep for RNA	Select Targets	Construct Library	Prepare Templates	Run sequence	NGS data analysis
PureLink™ RNA Mini Kit PureLink™ Viral RNA/DNA Mini Kit PureLink™ Pro 96 Viral RNA/DNA Purification Kit	Ion AmpliSeq Designer Oncomine™ assays Ion AmpliSeq NGS Panels for Targeted Sequencing	Ion Xpress™ Plus Fragment Library Ion Xpress™ Barcode Adapters 1-96 Kit Ion Library Equalizer™ Kit	Ion Chef System Ion AmpliSeq™ Kit for Chef DL8 Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef Ion 540™ Kit-Chef (2 sequencing runs per initialization)	Ion GeneStudio S5 Series sequencers Ion PGM Sequencer Ion Proton System	Torrent Suite Software for Sequencing Data Analysis

Ion AmpliSeq Panels for Targeted Sequencing

Simple, versatile technology based on highly multiplexed PCR helps you target tens to hundreds of genes across mutation types including SNPs, indels, fusions, and copy number variants. One tube simplicity to unravel complex biology associated with multiple areas of research: Cancer Research, Inherited Research, Transcriptome analysis, Human identification and work with any custom reference genome.

Run times and output on Ion GeneStudio S5 systems

Chip type	Number of reads	Read length (Output) †	Ion Gene Studio S5 System	Ion Gene Studio S5 Plus System	Ion Gene Studio S5 Prime System
Chip type	Number of reads	Read length (Output) †	Turnaround time (Sequencing run* and Analysis time)
Ion 510 Chip	2–3M	200 bp (0.3–0.5 Gb)	4.5 hr	3 hr	3 hr
Ion 510 Chip	2–3M	400 bp (0.6–1 Gb)	10.5 hr	5 hr	5 hr
Ion 520 Chip	4–6M	200 bp (0.6–1 Gb)	7.5 hr	3.5 hr	3 hr
	4–6M	400 bp (1.2–2 Gb)	12 hr	5.5 hr	5.5 hr
	3–4M	600 bp (0.5–1.5 Gb)	12 hr	5.5 hr	5.5 hr
Ion 530 Chip	15–20M	200 bp (3–4 Gb)	10.5 hr	5 hr	4 hr
	15–20M	400 bp (6-8 Gb)	21.5 hr	8 hr	6.5 hr
	9–12M	600 bp (1.5–4.5 Gb)	21 hr	8 hr	7 hr
Ion 540 Chip	60–80M	200 bp (10–15 Gb)	19 hr	10 hr	6.5 hr
Ion 540 Chip	60–80M	200 bp (20–30 Gb) 2 runs in 1 day	N/A	20 hr	10** hr
Ion 550 Chip	100–130M	200 bp (20–25 Gb)	N/A	11.5 hr	8.5 hr
Ion 550 Chip	100–130M	200 bp (40–50 Gb) 2 runs in 1 day	N/A	N/A	12** hr