Microbiology

The study of microorganisms, Microbiology, was predominantly based on culturing of organisms. Historically, growth of microorganisms in culture was used for identification of disease causing organisms. The incubate, inspect, identify and diagnose methodology is still a mainstay in hospital based microbial surveillance.

Besides identification of disease causing agents, bacteria such as E.coli, lactobacillus and yeast have become important as agents for cloning & sequencing, protein expression, probiotic augmentation and vehicles for bio-production of biological drug components.  Newer research in the realm of microbial interaction such as biofilms are adding tools in our arsenal to control bacterial contamination, understand quorum sensing and evolution of cell-cell interaction.

Solutions from

In this section we present basic microbiology culture and imaging solutions. Breakthrough technologies such as Metagenomics NGS analysis, & clinical diagnosis, food safety testing , veterinary sciences by real-time PCR or flow cytometry are addressed in the NGS, infectious disease and flow cytometry workflows.

Pathatrix™ Auto Instrument for Enrichment of Microbes

Pathatrix Auto Instrument

• Instrument and its companion kits facilitate concentration of bacteria such as Salmonella, E. coli O157:H7 and Listeria from up to 60 ml using RIMS (Re-Circulating Immuno-magnetic Separation)
• Associated Kits facilitate removal of PCR inhibitors affecting downstream enzymatic steps
• 10-60 mL sample volumes can be used per run
• Bacteria enriched are ideal for applications such as PCR, Lateral Flow, ELISA and plating on selective agar
• Pooling protocols validated by the AOAC Research Institute are available for Salmonella, E. coli O157:H7 and Listeria

---

Microbiological culture based Tests

Test methodology	Application	Instrument	Sector
Ames testing	Identification of mutagens using Salmonella typhimurium	ProtoCOL3	Environment & Water, Food & Drink, Pharmaceutical, Veterinary
Microbial limits test – pour plate	Estimate of viable microorganism in a sample	ProtoCOL3	Environment & Water, Food & Drink, Pharmaceutical, Veterinary
Microbial limits test – membrane filtration	Estimate of viable microorganism in a sample	ProtoCOL3	Environment & Water, Food & Drink, Pharmaceutical, Veterinary
Spiral plates	Estimate of viable microorganism in a sample	ProtoCOL3	Environment & Water, Food & Drink, Pharmaceutical, Veterinary
AST and microbial identification	Microbial ID	ChromoZona	Clinical
Coliform testing in Food	Safety testing	Protos 3, ProtoCOL3	Food & Drink
Preservative Efficacy Testing	Antimicrobial preservation of multiple-use cosmetic and pharmaceutical products	Protos 3, ProtoCOL3 aCOLyte 3 f	Pharmaceutical testing

Solutions for culture based studies

Published Works	Instrument	Application
Studies of cell density and protein expression in E.coli	SpectraMax M2 Multi-Mode Microplate Reader	Protein expression using fluorescence
Fluorescent bacterial colony selection Large scale DNA production Discovery of antimicrobials	QPix™ 400 Series	Bacterial colony pickers for high throughput screening
	Bioassay QTrays	Large scale culture and screening of bacteria
Bacterial identification	SpectraMax 190
Microarray printing and hybridization	QReps Colony replicators	Manual replication of Clone libraries
Biofilm analysis	VersaMax ELISA SpectraMax M3	Study of antimicrobials to penetrate & destroy biofilms

QPix 400 Series Microbial Colony Picker

SpectraMax i3 Multi-Mode Detection Platform

Adjustable Petri Dish and Microplate Holders

Microbial Growth Media and Consumables

Bacterial Growth Media	Liquid and powder growth media specifically formulated to culture bacteria for use in applications including cloning and protein expression
Bacterial Selection Antibiotics	Fast and potent selection antibiotics for use in bacterial culture
IPTG Induction	Most efficient inducing reagents for gene expression in bacteria
β-galactosidase Substrates	Commonly used substrates for measuring enzymatic activity of beta-galactosidase in bacteria
Yeast Growth Media	Liquid and powder growth media specifically formulated to culture yeast for use in maintaining and propagating yeast strains
Algae Growth Media	Gibco TAP Growth Media optimized for Chlamydomonas reinhardtii and Gibco BG-11 Growth Media optimized for Cyanobacteria

Bacterial Growth Media

LB Broth and LB Agar	Most referenced bacterial media for growth and maintenance of recombinant E. coli strains
Terrific Broth	Nutritionally rich bacterial media for higher density growth and maintenance of recombinant E. coli strains
M9 Minimal	Commonly used bacterial media for cultivation and maintenance of E. coli strains.
MagicMedia Medium	Customized bacterial media for growth of T7-regulated E. coli strains for auto induction of protein expression
ImMedia Medium	Pre-mixed, pre-sterilized bacterial media containing selection antibiotics for growth of E. colistrains
Other Media	Microbial media for outgrowth of transformed E. coli cells (S.O.C. Medium), solid microbial media (Invitrogen™ Select Agar™), and growth of hosts for replication of M13 vectors (2-YT Broth)

Microbial Consumables

Environmental	Sterilin™ Water Sampling Bottles Screw-Top Sterile Coliform Water Sample Bottle: Polystyrene, Polyethylene and Polypropylene
Clinical	MicroTest™ Tubes
Plates and Petridishes	Sterilin™ Standard 90mm Petri Dishes
Loops & Needles	Sterilin™ Loops and Spreaders
Swabs	BactiSwab™: Liquid Stuart Plastic Shaft
Microbiological consumables	Remel™ Easyplate Stacker
Atmosphere generation systems	AnaeroGen™, CampyGen™ and CO2Gen™ sachets and Co2 chambers

---

Microbiological Applications

Download PDF

Download PDF

Download PDF

Common Equipment

Equipment

Pipettes & tips

CO2 incubators

Water and dry baths

Lab hotplates and stirrers

Lab shakers

Vortex mixers

Lab heating and drying ovens

Vacuum concentrators

Water purification

Centrifuges

Cold Storage

Plastic

Bottles and accessories

Tubes, Vials and Racks

Centrifuge tubes

Microplates, Storage plates and accessories

Filters

General Plasticware