Gene Expression (GE) is a method to compare expression levels of mRNA, rRNA or small RNA between two samples (two different experimental conditions, disease). Differential expression of genes between samples can be studied using real-time PCR, Digital PCR, Transcriptome Analysis by Next Generation Sequencing (NGS). Gene Expression is a critical tool for many applications in Cancer research, copy number variation analysis, downstream of gene editing and silencing, Viral load analysis and several others.
Our offerings for Gene expression are:
- Gene Expression Analysis by Sequencing
- Gene Expression analysis by Real-time PCR
- Gene Expression quantification by Digital PCR
- Common Equipment
Transcriptome sequencing by next-generation sequencing enables the discovery of differentially expressed genes using a hypothesis-neutral approach. Sanger sequencing methods can also be used for gene expression profiling and analysis.
Real-time PCR is the gold-standard technique for validation of differential gene expression profiles. Our comprehensive offering of TaqMan Gene Expression Assays and flexible formats enable fast and cost-effective results.
Digital PCR enables highly precise measurements and is able to resolve gene expression level changes of two-fold or less. Digital PCR can also be used to determine the absolute quantification of a transcript without the need for a reference gene.