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FLOW CYTOMETRY

Flow cytometry is a technique that allows analysis of cell parameters such as cell dimensions, expression of proteins in a cell and others. These cell measurements (up to 30 unique parameters) are taken using fluorescent measurements as the cell flows past a detection system. Protein expression-throughout the entire cell, even the nucleus.

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Potential applications of Flow Cytometry include:

  • Protein post translational modifications-includes cleaved and phosphorylated proteins
  • RNA including IncRNA, miRNA, and mRNA transcripts
  • Cell health status from viability to late stage apoptosis or programmed cell death
  • Cell cycle status providing a powerful tool to assess cells in G0/G1 phase versus S phase, G2, or polyploidy, including analysis of cell proliferation and activtation
  • Identification and characterization of distinct subsets of cells within a heterogeneous sample incluing distinguishing central effector memory cells from exhausted T cells or even regulatory T cells

Flow cytometer requires a suspension of single cells. There are 3 main components of a flow cytometer the fluidics, optics and electronics that work together to provide a complete system of cell analysis. Flow cytometers use hydrodynamic focusing or acoustic assisted hydrodynamic focusing to control the flow of the cells through the interrogation point (point where cell interacts with the optics and data is collected), allowing more precise data collection.

Read more here on how a Flow cytometer works

Additional information on assays and reagents for Flow cytometry experiments:

Antibodies for Flow Cytometry
Apoptosis Assays for Flow Cytometry
Cell Cycle Assays for Flow Cytometry
Cell Proliferation Using Flow Cytometry
Cell Viability Assays for Flow Cytometry
Custom Antibody Services
Flow Cytometer Calibration and Size Reference Beads
Flow Cytometer Cell Counting Beads
Flow Cytometry Compensation Beads
Super Bright polymer dyes eFluor organic dyes

Common Equipment

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