Forensics

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Forensic Workflow

DNA based identification and characterization is one of the very important discoveries of modern biological science. The DNA based identification is a very powerful tool and its use in various field of biological sciences has an immense effect to the research and development. One of the field which has got tremendous benefit from this tool is the forensic sciences for its role in the investigation and prosecution of crime. Innovative technologies along with the automation has been proven very useful in crime investigation. DNA based methods can be used in solving the criminal cases and creating the national database. The Next generation sequencing (NGS) technology has added additional advantages for being High throughput and challenging samples can be processed with higher confidence. We are offering complete range of product for the all your forensic application from low to high throughput.

Solutions from

Case Work Samples
National DNA Database
Next generation sequencing technology

Workflow for Casework samples for Low to medium throughput labs

We offer complete workflow for high-quality, consumables, and assay solutions designed to meet your needs, whether you belong to a smaller lab or a higher-throughput lab for solving crimes. The Automate Express enables you to perform Medium to Low throughput DNA extraction and the subsequent steps of STR analysis using different loci kits ranging from 9 to 24 loci is used to genotypically characterize an individual.

DNA Extraction	DNA Quantification	Amplification	Capilarry Electrophoresis	Data Analysis
Manual Automate Express	Quantifiler Human Quantifiler HP Quantifiler Duo Quantifiler Trio Real Time PCR	Identifiler Plus Kit Identifiler Direct kit GlobalFiler Kit GlobalFiler express kit Veriti Thermal Cycler	3500 Genetic Analyzer 3500 XL Genetic Analyzer SeqStudio	GMID X software Converge Forensic analysis Software

Step1 DNA Extraction

Manual

Using Robust PrepFiler kits which removes inhibitors effectively to yield pure DNA free from Inhibitors. For more information please visit : https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Automated

For a medium to low throughput labs we offer a simple automated low throughput DNA extraction system for extracting 13 sample including a control. The PrepFiler chemistry especially design for this system, packaged in pre-filled, foil sealed cartridges. The chemistry can extract DNA from almost all the forensic samples and with BTA buffer can also effectively extract DNA from bone and teeth. The extracted DNA can further be used for the downstream STR applications. Due to less manual intervention the chances of contamination is less. For more information please visit https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Step2 DNA Quantitation by RTPCR using Quantifiler kits

There is always uncertainty for the DNA quantity in the forensic sample and for the economical reason DNA Quantification is an essential step to decide whether to proceed for the next step. The amount of DNA present in the sample will provide the vital information processing the samples further. We offer from Thermo verity of Real Time PCR based DNA Quantification kits to take the right decision for the next step in the workflow. Choose from below the kits that suits the requirement for the particular case. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-quantification-solutions.html

Step3 DNA Amplification

Short tandem repeat (STR) analysis is powerful tool used by forensic analysis that evaluates specific STR regions found on nuclear DNA. The variable (polymorphic) nature of the STR regions that are analyzed for forensic testing intensifies the discrimination between one DNA profile and another. Forensic science takes advantage of the population’s variability in STR lengths, enabling scientists to distinguish one DNA sample from another. Thermofisher is a leading brand in the field of Forensic DNA identification and offer variety of AmpFℓSTR™ kits. These kits combine the sensitivity of PCR chemistry with the power of multicolor fluorescence technology to enable robust, reliable results for all human identification applications-even with mixed, degraded, and challenged samples. Many DNA casework and database laboratories have been built using AmpFℓSTR™ kits;. For more information please visit https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling.html

Step4 Capillary Electrophoresis:

Applied Biosystems has more than 30 years of expertise in the field of Sequencing and Fragment analysis and is world leader in capillary electrophoresis. The 3500 series come in two formats 8 capillaries and 24 capillaries and designed with specific features to support the human identification application. The complete product combines the instrument with Applied Biosystems^® reagents, consumables and software, as well as IGB leading onsite support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies.
The New SeqStudio from Applied Biosystems is a low throughput easy to use system to generate the DNA profile and can be used effectively in any low throughput forensic lab. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis.html

Step5 Data Analysis

Thermofisher integrated solution for the data analysis of forensic sample can help streamline and simplify your lab operation. For more information about the software offer please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/forensic-dna-data-interpretation.html

Workflow for Casework samples for High throughput labs

Automation has become an indispensable tool in forensic laboratories challenged with ever increasing workloads and the pressure to reduce turnaround times. Forensic DNA analysis is a multi-step process involving extraction of DNA, quantification of human DNA, amplification using multiplex STR systems, separation of amplified products, and data analysis. We describe here an automated forensic sample processing station, the AutoLys and Startlet ID System, based on the Hamilton robotic platform, which automates DNA extraction, setup of qPCR to quantify human DNA, DNA dilution to normalize input amount, and setup of PCR reactions for amplification of STR multiplexes.

Autolysis	DNA Extraction	DNA Quantification	Amplification	Capilarry Electrophoresis	Data Analysis
Hamilton Autolys	Starlet ID PepFiler Automated Forensic extraction kit	Quantifiler Human Quantifiler HP Quantifiler Duo Quantifiler Trio Real Time PCR	Identifiler Plus Kit Identifiler Direct kit GlobalFiler Kit GlobalFiler express kit Veriti Thermal Cycler	3500 Genetic Analyzer 3500 XL Genetic Analyzer	GMID X software Converge Forensic analysis Software

Step1 Autolys:

The Microlab Starlet Autolys developed by Hamilton Robotics automate the full process of lysing majority of the forensic samples e.g. dried blood, saliva stains, Cigarette butts, stains on denim etc. The unique AutoLys tube carries out hands-free, uninterrupted preparation of cleared lysates separated from solid material. Additionally, the Autolys workstation can be configured to automate the complete process from raw sample lysis through DNA purification using the Starlet ID. For more detail please visit: https://www.hamiltoncompany.com/products/automated-liquid-handling/standard-solutions/autolys-star

Step2 Automated DNA Extraction

ID Starlet, a unique device developed by Hamilton Robotics especially for the forensic workflow. Its streamlined the forensic workflow in fully automated with very less of human interference. The system carry out all the pre-PCR steps from DNA purification, RTPCR Setup for the DNA quantification and normalization to setting up the PCR plate for the amplification. The DNA will be extracted using Prepfiler Automated Forensic kit. For more detail please visit: https://www.hamiltoncompany.com/products/automated-liquid-handling/standard-solutions/id-starlet
https://www.thermofisher.com/order/catalog/product/4463353?SID=srch-srp-4463353

Step3 DNA Quantitation by RTPCR using Quantifiler kits

There is always uncertainty for the DNA quantity in the forensic sample and for the economical reason DNA Quantification is an essential step to decide whether to proceed for the next step. The amount of DNA present in the sample will provide the vital information processing the samples further. We offer from Thermo verity of Real Time PCR based DNA Quantification kits to take the right decision for the next step in the workflow. Choose from below the kits that suits the requirement for the particular case. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-quantification-solutions.html

Step4 DNA Amplification

Short tandem repeat (STR) analysis is a powerful tool used by forensic analysis that evaluates specific STR regions found on nuclear DNA. The variable (polymorphic) nature of the STR regions that are analyzed for forensic testing intensifies the discrimination between one DNA profile and another. Forensic science takes advantage of the population’s variability in STR lengths, enabling scientists to distinguish one DNA sample from another. Thermofisher is a leading brand in the field of Forensic DNA identification and offers variety of AmpFℓSTR™ kits. These kits combine the sensitivity of PCR chemistry with the power of multicolor fluorescence technology to enable robust, reliable results for all human identification applications-even with mixed, degraded, and challenged samples. Many DNA casework and database laboratories have been built using AmpFℓSTR™ kits. For more information please visit https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling.html

Step5 Capillary Electrophoresis:

Applied Biosystems has more than 30 years of expertise in the field of Sequencing and Fragment analysis and is the world leader in capillary electrophoresis. The 3500 series come in two formats 8 capillaries and 24 capillaries and designed with specific features to support the human identification application. The complete product combines the instrument with Applied Biosystems^®(≥ 50%) reagents, consumables and software, as well as IGB leading onsite support, to provide an integrated HID system solution that significantly improves ease-of-use and application efficiencies
The New SeqStudio from Applied Biosystems is a low throughput easy to use system to generate the DNA profile and can be used effectively in any low throughput forensic lab. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis.html

Step6 Data Analysis

Thermofisher integrated solution for the data analysis of forensic sample can help streamline and simplify your lab operation. For more information about the software offer please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/forensic-dna-data-interpretation.html

Sexual Assault Cases

Sexual assaults and other violent assault cases represent a significant percentage of cases examined by crime laboratories (≥50%). DNA analysis and interpretation can be challenging, given the complex nature of sexual assault evidence. Samples often consist of mixtures of body fluids from the victim and suspect, and sometimes contain small quantities of DNA, highly degraded DNA, or inhibitors. The DEPArray™ system resolves this uncertainty by enabling forensic laboratory scientists to separate blood cells, from epithelial cells, from sperm cells, with 100% precision. Such separation, prior to genetic analysis, allows distinct profiling of multiple individual contributors by routine DNA identification methods.

Epithelium and Sperm Cell Separation	DNA Extraction	DNA Quantification	Amplification	Capilarry Electrophoresis	Data Analysis
DEParray Nxt to separate Epithelial and Sperm Cells	Manual Automate Express	Quantifiler Duo Quantifiler Trio Real Time PCR	Quantifiler Human Quantifiler HP Quantifiler Duo Quantifiler Trio Real Time PCR	3500 Genetic Analyzer 3500 XL Genetic Analyzer SeqStudio

Step1 Epithelial and Sperm Cell Separation

The DEPArray™ system enables the forensic laboratory scientists to separate blood cells, from epithelial cells, from sperm cells, with 100% precision. Such separation, prior to genetic analysis, allows distinct profiling of multiple individual contributors by routine DNA identification methods. For more information please visit:;
http://www.siliconbiosystems.com/forensics

Step2 DNA Extraction

Manual

Using Robust PrepFiler kits which removes inhibitors effectively to yield pure DNA free from Inhibitors. For more information please visit: https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Automated

Using PrepFiler Express extraction kit on Automate express platform. For more information please visit: https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Step3 DNA Quantitation by RTPCR usinf Quantifiler kits

Quantifying the DNA in Forensic laboratory is very important step for the downstream application. The Quantifiler Duo and Trio kits will assist the analyst for the quality and Quantity of the DNA. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-quantification-solutions.html

Step4 DNA Amplification

The AmpFlSTR kits will be used for the amplification and profiling of the STR. Many DNA casework and database laboratories have been built using AmpFℓSTR™ kits. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/pcr-amplification-forensic-dna-profiling.html

Step5 Capillary Electrophoresis

The Applied Biosystems 3500 Series genetic analyzers are widely used for forensic DNA analysis. They offer superior data quality, variable throughput support, and reagent tracking.
The New SeqStudio from Applied Biosystems is a low throughput easy to use system to generate the DNA profile and can be used effectively in any low throughput forensic lab. For more information please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis.html

Step6 Data Analysis

Thermofisher integrated solution for the data analysis of forensic sample can help streamline and simplify your lab operation. For more information about the software offer please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/forensic-dna-data-interpretation.html

Workflow for National DNA Database (High throughput labs)

A national DNA database is a government database of DNA profiles which can be used by law enforcement agencies to identify suspects of crimes. DNA technology has proved to be a valuable investigative tool which has helped to exonerate the innocent and to bring those responsible for serious crimes to justice. We offer a complete fully automated workflow with Easy Punch and Starlet ID platform from Hamilton to automate the full process. Direct amplification can dramatically increase your lab’s throughput and simultaneously decrease the complexity of your STR analysis processes. This reduces costs and minimizes the potential for errors. The Identifiler Direct or Globalfiler Express kits optimized protocols without the need for sample purification, for blood or saliva on Copan Cards.

Sample Collection	Sample Punching with esypunch	Direct Amplification	Capilarry Electrophoresis	Data Analysis
Copan Sample Collection Kit Buccal/Blood	Easy Punch from Hamilton	Identifiler Direct kit GlobalFiler express kit Veriti Thermal Cycler	3500 Genetic Analyzer 3500 XL Genetic Analyzer	GMID X software Converge Forensic analysis Software

Step1 Sample Collection

We offer a very easy and convenient sample collection card from Copan to collect either blood or buccal sample for DNA extraction and the downstream analysis. The card is chemically treated to perform the lysis of the cell and protein denaturation. The nucleic acid will be immobilize on the card and preserved for long-term storage at room temperature. NUCLEIC-CARD™ COLOR changes color upon sample application to facilitate processing of buccal samples. NUCLEIC-CARD™ collection devices are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

Step2 Card Puncing using EasyPunch

Partnered with one of the world’s best Robotic system Hamilton Company IGB offer a very comprehensive instrument in the form of easyPunch to prepare PCR master mix punch the samples from the Copan card and dispense in the PCR master mix. Its flexible modular nature makes it easy to tailor the workflow exactly to your needs. For more information please visit: https://www.hamiltoncompany.com/products/automated-liquid-handling/standard-solutions/easypunch-starlet

Step3 Direct Amplification

The databasing lab needs fast turnaround time to accomplish goals. The Identifiler Direct and Globafiler express kits from Applied Biosystems offer the direct amplification without extracting the DNA sample for 16 and 24 loci respectively. These kits still have the same sensitivity and reproducibility as Identifiler kit. This kit minimize the extraction cost and also helps reduce the cost of labor and consumables. For more information please visit: https://www.thermofisher.com/order/catalog/product/4467831?SID=srch-srp-4467831
https://www.thermofisher.com/order/catalog/product/4476609?SID=srch-srp-4476609

Step4 Capillary Electrophoresis

The Applied Biosystems 3500 Series genetic analyzers are widely used for forensic DNA analysis. They offer superior data quality, variable throughput support, and reagent tracking.
https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis.html

Step5 Data Analysis

Thermofisher integrated solution for the data analysis of forensic sample can help streamline and simplify your lab operation. For more information about the software offer please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/forensic-dna-data-interpretation.html

Next generation sequencing technology

Next Generation sequencing is proving the major breakthrough in the field of DNA sequencing and many forensic laboratories are also adopting to this new technology. The NGS is playing pivotal role for the forensic laboratories for solving the cases where the samples are challenging. Adopting next-generation sequencing (NGS) for forensic DNA analysis in your laboratory is simpler than ever, when you combine the Ion Chef System and Ion S5 or Ion S5 XL Systems with optimized Precision ID library preparation, template preparation and sequencing kits, and forensically relevant panels. For more information for NGS in Forensic application please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis/next-generation-sequencing-ngs-forensics.html

mtDNA Sequencing Ancestry Panel Identity Panel

mtDNA Sequencing

Mitochondrial DNA is present in rootless shed hairs, hair fragments, skeletal remains, and other samples too minimal and/or degraded for STR testing. mtDNA testing is useful tool in forensic for investigating in crime scene, missing persons, genealogical investigation, adoption maternity, historical cases, cold cases, ancient DNA, and post-conviction evaluation. We offer complete 16K mtDNA sequence analysis with multiplexing of the sample at an affordable price with Ion Torrent technology with Precision ID mtDNA Whole Genome Panel. A complete workflow from Library preparation to sequence analysis using ion S5 and Ion Chef.

mtDNA Extraction Manual/Automated	Ampliseq Library Preparation	Template Preparation using OneTouch2 Or Ion Chef System	Sequencing Using S5 or PGM	Data Analysis
PrepFiler DNA Extraction kit	Manual- Precision ID Library Kit Ion Chef-Precision ID DL8 Kit	Ion Chef- Ion Chef Template kit	Ion PGM – 314, 316 and 318 chip S5- 510, 520, 530, 540 chip	Ion Reporter server

Step1 DNA Extraction

Manual

Using Robust PrepFiler kits which removes inhibitors effectively to yield pure DNA free from Inhibitors. For more information please visit: https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Automated

Using PrepFiler Express extraction kit on Automate express platform. For more information please visit: https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Step2 DNA Quantitation Using Qubit

Invitrogen Qubit fluorometric quantitation comprises the easy-to-use Invitrogen Qubit 4 Fluorometer and sensitive, specific Invitrogen Qubit quantitation assays to measure RNA quality and quantity, quantitate RNA, ssDNA, dsDNA and protein for applications that require sensitive, specific and accurate measurement. For more information please visit:

https://www.thermofisher.com/order/catalog/product/Q33228?SID=srch-srp-Q33228

Step3 DNA Amplification and Library Preparation

The Panel contains set of primers to amplify whole mtDNA using the primer pool. The DL8 kit for automated library preparation gives an advantage of full automation without manual intervention for 8 samples.
For more information please visit: https://www.thermofisher.com/order/catalog/product/A26435
For Automated Library preparation use the Ion Chef platform. For more information please visit: https://www.thermofisher.com/order/catalog/product/A33212

Step4 Template Preparation

The Ion Chef provides the user for the complete automated of templated bead preparation. The templated beads will be performed by emulsion PCR and after the template preparation the chips will be ready to be processed for sequencing using PGM or Ion S5 sequencers.
https://www.thermofisher.com/order/catalog/product/4484177?SID=srch-srp-4484177
https://www.thermofisher.com/search/results?query=ion+530%E2%84%A2+kit-chef&persona=Catalog&navId=10975&resultPage=1&refinementAction=true

Step5 Sequencing

PGM- The Ion PGM systems combines the efficiency and reliability with faster turn around time. The first post light instrument is easy to operate and maintained. The sequencer is good for sequencing targeted panels, small microbial genomes and miRNA. For more information please visit: https://www.thermofisher.com/order/catalog/product/4462921?SID=srch-srp-4462921
S5 and S5XL- Powered by the first post light Ion torrent technology, the Ion S5 and S5XL system provides the user the minimum hands on time to load the sequences. Combined with IC From sample to results, there are no centrifugation steps, and less pipetting steps and hands on time of the alternative NGS workflow.
https://www.thermofisher.com/order/catalog/product/A27214?SID=srch-srp-A27214
https://www.thermofisher.com/order/catalog/product/A27212?SID=srch-srp-A27212

Step6 Data Analysis

Ion reporter Server- The Ion Reporter Server System is a combined hardware and software solution for analyzing human and bacterial sequencing data. It consists of a locally deployed computer with a suite of informatics tools that streamlines and simplifies analysis, annotation, and archiving of semiconductor sequencing data from any Ion Torrent sequencer.
https://www.thermofisher.com/order/catalog/product/4487118?SID=srch-srp-4487118

Ancestry Panel

The Applied Biosystems™ Precision ID Ancestry Panel includes 165 autosomal markers (SNPs) that can provide biogeographic ancestry information to guide your investigative process. Fifty-five of these markers were selected based on a publication by Dr. Kenneth Kidd from Yale University [1], and 123 markers were selected based on a publication by Dr. Michael Seldin from the University of California Davis [2]. With small amplicon sizes (<130 bp), this panel is also optimized for degraded DNA samples. Using a small sample input of as little as 1 ng of DNA, you can go from sample extraction to genotypes in less than a two days using the Precision ID NGS System and HID SNP Genotyper plug-in for analysis.

DNA Extraction Manual/Automated	Ampliseq Library Preparation	Ion Chef System	Sequencing Using S5 or PGM	Data Analysis
PrepFiler DNA Extraction kit	Manual- Precision ID Identity Panel Ion Chef-Precision ID DL8 Kit	Ion Chef- Ion Chef Template kit	Ion PGM – 314, 316 and 318 chip S5- 510, 520, 530, 540 chip	Ion Reporter server

References:1. Kidd et. al. Poster: Better SNPs for Better Forensics: Ancestry, Phenotype, and Family Identification. Shown at National Institute of Justice annual meeting, Arlington VA, June 2012.
2. Kosoy R, Nassir R, Tian C, et al. (2009) Ancestry informative marker sets for determining continental origin and admixture proportions in common populations in America. Hum Mutat 30(1) 69–78.

Step1 DNA Extraction

Manual

Using Robust PrepFiler kits which removes inhibitors effectively to yield pure DNA free from Inhibitors. For more information please visit: https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

– Automated

Using PrepFiler Express extraction kit on Automate express platform. For more information please visit: https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Step2 DNA Quantitation Using Qubit

https://www.thermofisher.com/order/catalog/product/Q33228?SID=srch-srp-Q33228

Step3 Amplification and Library Preparation

The Panel contains set of primers to amplify the specified region of the DNA to detect specific SNP for the sample identification. The DL8 kit for automated library preparation gives an advantage of full automation without manual intervention for 8 samples. For more information please visit: https://www.thermofisher.com/order/catalog/product/A26435
https://www.thermofisher.com/order/catalog/product/A25642
For Automated Library preparation use the Ion Chef platform. For more information please visit:
https://www.thermofisher.com/order/catalog/product/A33212

Step4 Template Preparation

The Ion Chef provides the user for the complete automated of templated bead preparation. The templated beads will be performed by emulsion PCR and after the template preparation the chips will be ready to be processed for the
https://www.thermofisher.com/order/catalog/product/4484177?SID=srch-srp-4484177
https://www.thermofisher.com/search/results?query=ion+530%E2%84%A2+kit-chef&persona=Catalog&navId=10975&resultPage=1&refinementAction=true

Step5 Sequencing

Step6 Data Analysis

Ion reporter Server
https://www.thermofisher.com/order/catalog/product/4487118?SID=srch-srp-4487118

Identity Panel

The Applied Biosystems™ Precision ID Identity Panel is one of the first next-generation sequencing (NGS) solutions for human identification that uniquely identifies pristine or degraded samples. High discrimination power is achieved by using 34 upper Y-Clade SNPs and 90 autosomal SNPs that have high heterozygosity and low fixation index (Fst) as described in publications by Dr. Kenneth Kidd from Yale University and the SNPForID Consortium.

DNA Extraction Manual/Automated	Ampliseq Library Preparation	Ion Chef System	Sequencing Using S5 or PGM	Data Analysis
PrepFiler DNA Extraction kit	Manual- Precision ID Identity Panel Ion Chef-Precision ID DL8 Kit	Ion Chef- Ion Chef Template kit	Ion PGM – 314, 316 and 318 chip S5- 510, 520, 530, 540 chip	Ion Reporter server

Step1 DNA Extraction

Manual

Using Robust PrepFiler kits which removes inhibitors effectively to yield pure DNA free from Inhibitors. For more information please visit : https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Automated

Using PrepFiler Express extraction kit on Automate express platform. For more information please visit https://www.thermofisher.com/order/catalog/product/4441352?SID=srch-srp-4441352

Step2 DNA Quantitation Using Qubit

https://www.thermofisher.com/order/catalog/product/Q33228?SID=srch-srp-Q33228

Step3 DNA Amplification and Library Preparation

The Panel contains set of primers to amplify the specified region of the DNA to detect specific SNP for the sample identification. The DL8 kit for automated library preparation gives an advantage of full automation without manual intervention for 8 samples. For more information please visit:
https://www.thermofisher.com/order/catalog/product/A26435
https://www.thermofisher.com/order/catalog/product/A25643
Automated Library Preparation using IC
For Automated Library preparation use the Ion Chef platform. For more information please visit:
https://www.thermofisher.com/order/catalog/product/A33212

Step4 Template Preparation

The Ion Chef provides the user for the complete automated of templated bead preparation. The templated beads will be performed by emulsion PCR and after the template preparation the chips will be ready to be processed for the
https://www.thermofisher.com/order/catalog/product/4484177?SID=srch-srp-4484177
https://www.thermofisher.com/search/results?query=ion+530%E2%84%A2+kit-chef&persona=Catalog&navId=10975&resultPage=1&refinementAction=true

Step5 Sequencing

Step6 Data Analysis

Ion reporter Server
https://www.thermofisher.com/order/catalog/product/4487118?SID=srch-srp-4487118

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Forensics

Forensic Workflow

Workflow for Casework samples for Low to medium throughput labs

Step1 DNA Extraction

Step2 DNA Quantitation by RTPCR using Quantifiler kits

Step3 DNA Amplification

Step4 Capillary Electrophoresis:

Step5 Data Analysis

Workflow for Casework samples for High throughput labs

Step1 Autolys:

Step2 Automated DNA Extraction

Step3 DNA Quantitation by RTPCR using Quantifiler kits

Step4 DNA Amplification

Step5 Capillary Electrophoresis:

Step6 Data Analysis

Sexual Assault Cases

Step1 Epithelial and Sperm Cell Separation

Step2 DNA Extraction

Step3 DNA Quantitation by RTPCR usinf Quantifiler kits

Step4 DNA Amplification

Step5 Capillary Electrophoresis

Step6 Data Analysis

Workflow for National DNA Database (High throughput labs)

Step1 Sample Collection

Step2 Card Puncing using EasyPunch

Step3 Direct Amplification

Step4 Capillary Electrophoresis

Step5 Data Analysis

Next generation sequencing technology

mtDNA Sequencing

Step1 DNA Extraction

Step2 DNA Quantitation Using Qubit

Step3 DNA Amplification and Library Preparation

Step4 Template Preparation

Step5 Sequencing

Step6 Data Analysis

Ancestry Panel

Step1 DNA Extraction

Step2 DNA Quantitation Using Qubit

Step3 Amplification and Library Preparation

Step4 Template Preparation

Step5 Sequencing

Step6 Data Analysis

Identity Panel

Step1 DNA Extraction

Step2 DNA Quantitation Using Qubit

Step3 DNA Amplification and Library Preparation

Step4 Template Preparation

Step5 Sequencing

Step6 Data Analysis

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