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Forensics

Forensic Workflow

DNA based identification and characterization is one of the very important discoveries of modern biological science. The DNA based identification is a very powerful tool and its use in various field of biological sciences has an immense effect to the research and development. One of the field which has got tremendous benefit from this tool is the forensic sciences for its role in the investigation and prosecution of crime. Innovative technologies along with the automation has been proven very useful in crime investigation. DNA based methods can be used in solving the criminal cases and creating the national database. The Next generation sequencing (NGS) technology has added additional advantages for being High throughput and challenging samples can be processed with higher confidence. We are offering complete range of product for the all your forensic application from low to high throughput.

Solutions from

  • Case Work Samples
  • National DNA Database
  • Next generation sequencing technology

Next generation sequencing technology

Next Generation sequencing is proving the major breakthrough in the field of DNA sequencing and many forensic laboratories are also adopting to this new technology. The NGS is playing pivotal role for the forensic laboratories for solving the cases where the samples are challenging. Adopting next-generation sequencing (NGS) for forensic DNA analysis in your laboratory is simpler than ever, when you combine the Ion Chef System and Ion S5 or Ion S5 XL Systems with optimized Precision ID library preparation, template preparation and sequencing kits, and forensically relevant panels. For more information for NGS in Forensic application please visit: https://www.thermofisher.com/sa/en/home/industrial/forensics/human-identification/forensic-dna-analysis/dna-analysis/next-generation-sequencing-ngs-forensics.html

mtDNA SequencingAncestry PanelIdentity Panel

mtDNA Sequencing

Mitochondrial DNA is present in rootless shed hairs, hair fragments, skeletal remains, and other samples too minimal and/or degraded for STR testing. mtDNA testing is useful tool in forensic for investigating in crime scene, missing persons, genealogical investigation, adoption maternity, historical cases, cold cases, ancient DNA, and post-conviction evaluation. We offer complete 16K mtDNA sequence analysis with multiplexing of the sample at an affordable price with Ion Torrent technology with Precision ID mtDNA Whole Genome Panel. A complete workflow from Library preparation to sequence analysis using ion S5 and Ion Chef.

mtDNA Extraction Manual/Automated Ampliseq Library Preparation Template Preparation using OneTouch2 Or Ion Chef System Sequencing Using S5 or PGM Data Analysis
  • PrepFiler DNA Extraction kit
  • Manual- Precision ID Library Kit Ion Chef-Precision ID DL8 Kit
  • Ion Chef- Ion Chef Template kit
  • Ion PGM – 314, 316 and 318 chip S5- 510, 520, 530, 540 chip
  • Ion Reporter server



Step1 DNA Extraction

Manual

Automated

Step2 DNA Quantitation Using Qubit

Invitrogen Qubit fluorometric quantitation comprises the easy-to-use Invitrogen Qubit 4 Fluorometer and sensitive, specific Invitrogen Qubit quantitation assays to measure RNA quality and quantity, quantitate RNA, ssDNA, dsDNA and protein for applications that require sensitive, specific and accurate measurement. For more information please visit:

Step3 DNA Amplification and Library Preparation

Step4 Template Preparation

Step5 Sequencing

Step6 Data Analysis

  • Ion reporter Server- The Ion Reporter Server System is a combined hardware and software solution for analyzing human and bacterial sequencing data. It consists of a locally deployed computer with a suite of informatics tools that streamlines and simplifies analysis, annotation, and archiving of semiconductor sequencing data from any Ion Torrent sequencer.
  • https://www.thermofisher.com/order/catalog/product/4487118?SID=srch-srp-4487118

Ancestry Panel

The Applied Biosystems™ Precision ID Ancestry Panel includes 165 autosomal markers (SNPs) that can provide biogeographic ancestry information to guide your investigative process. Fifty-five of these markers were selected based on a publication by Dr. Kenneth Kidd from Yale University [1], and 123 markers were selected based on a publication by Dr. Michael Seldin from the University of California Davis [2]. With small amplicon sizes (<130 bp), this panel is also optimized for degraded DNA samples. Using a small sample input of as little as 1 ng of DNA, you can go from sample extraction to genotypes in less than a two days using the Precision ID NGS System and HID SNP Genotyper plug-in for analysis.

DNA Extraction Manual/Automated Ampliseq Library Preparation Ion Chef System Sequencing Using S5 or PGM Data Analysis
  • PrepFiler DNA Extraction kit
  • Manual- Precision ID Identity Panel Ion Chef-Precision ID DL8 Kit
  • Ion Chef- Ion Chef Template kit
  • Ion PGM – 314, 316 and 318 chip S5- 510, 520, 530, 540 chip
  • Ion Reporter server



References:1. Kidd et. al. Poster: Better SNPs for Better Forensics: Ancestry, Phenotype, and Family Identification. Shown at National Institute of Justice annual meeting, Arlington VA, June 2012.
2. Kosoy R, Nassir R, Tian C, et al. (2009) Ancestry informative marker sets for determining continental origin and admixture proportions in common populations in America. Hum Mutat 30(1) 69–78.

Step1 DNA Extraction

Manual

– Automated

Step2 DNA Quantitation Using Qubit

Step3 Amplification and Library Preparation

Step4 Template Preparation

Step5 Sequencing

Step6 Data Analysis


Identity Panel

The Applied Biosystems™ Precision ID Identity Panel is one of the first next-generation sequencing (NGS) solutions for human identification that uniquely identifies pristine or degraded samples. High discrimination power is achieved by using 34 upper Y-Clade SNPs and 90 autosomal SNPs that have high heterozygosity and low fixation index (Fst) as described in publications by Dr. Kenneth Kidd from Yale University and the SNPForID Consortium.

DNA Extraction Manual/Automated Ampliseq Library Preparation Ion Chef System Sequencing Using S5 or PGM Data Analysis
  • PrepFiler DNA Extraction kit
  • Manual- Precision ID Identity Panel Ion Chef-Precision ID DL8 Kit
  • Ion Chef- Ion Chef Template kit
  • Ion PGM – 314, 316 and 318 chip S5- 510, 520, 530, 540 chip
  • Ion Reporter server



Step1 DNA Extraction

Manual

Automated

Step2 DNA Quantitation Using Qubit

Step3 DNA Amplification and Library Preparation

Step4 Template Preparation

Step5 Sequencing

Step6 Data Analysis


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