Absolute Q Digital PCR

Delivering precision and speed to put you above the curve

The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR (dPCR) platform powered by proprietary microfluidic array plate (MAP) technology. that enables all the necessary steps for dPCR—compartmentalizing, thermal cycling, and data acquisition—to be conducted on a single instrument. The dPCR workflow is identical to the qPCR workflow you are familiar with to improve ease of use, minimize hands-on steps, and maximize consistency.

Workflow

dPCR overcomes variability and low accuracy by eliminating the need for a standard curve. The QuantStudio Absolute Q Digital PCR System consists of just one instrument, consolidating all steps required into a single plate and transforming a multi-step multi-instrument workflow into a one-step qPCR-like workflow.

Features

An instrument is only as good as the reagents you use with it. Learn more about our Absolute Q digital PCR master mix, specifically designed for use with the QuantStudio Absolute Q and developed for optimal multiplexing performance and digital PCR sensitivity. TaqMan real-time PCR assays have long been considered the gold standard for genetic analysis. Now, using our decades of experience, unique assay design algorithms, and wet-lab verification process, we have developed a portfolio of equally robust Absolute Q dPCR assays. And, just like our TaqMan Assays, the Absolute Q dPCR assays are backed by a performance guarantee.

Single instrument. Single plate. Simple workflow.

Eliminate cumbersome and challenging sample and reagent preparation steps from your dPCR workflow. The QuantStudio Absolute Q system requires only one hands-on step that takes under five minutes to complete with minimal technical skill.

Industry-leading consistency

Powered by microfluidic array plate (MAP) technology, the QuantStudio Absolute Q system and MAP16 dPCR plates enable exceptional consistency in total micro-chambers analyzed.

Minimize wasted sample volume

Leveraging MAP technology and dead-ended reagent loading, over 95% of your input sample is analyzed per reaction compared to the 25–60% of other dPCR platforms.

Fast time to answer

Avoid wasting precious time moving generating micro-chambers, carrying plates, and reading samples. In under 90 minutes* reagent digitization, thermal cycling, and data collection are integrated into a single system with no manual transfer steps.

*90-minute time includes reagent digitization, thermal cycling, and data collection. The total time may vary based on assay-specific thermal cycling requirements.

• Key Applications – Rare target quantification, Genotyping, Gene editing, Copy number variation Absolute quantification
• Research Areas – Oncology, Reproductive health, Infectious disease, Gene Editing & Inherited disease.

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