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Absolute Q Digital PCR

Digital PCR is a method of quantifying nucleic acid targets without standard curves by dividing the bulk reaction into thousands of smaller, independent reactions.

Delivering precision and speed to put you above the curve

The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR (dPCR) platform powered by proprietary microfluidic array plate (MAP) technology. that enables all the necessary steps for dPCR—compartmentalizing, thermal cycling, and data acquisition—to be conducted on a single instrument. The dPCR workflow is identical to the qPCR workflow you are familiar with to improve ease of use, minimize hands-on steps, and maximize consistency.

Workflow

dPCR overcomes variability and low accuracy by eliminating the need for a standard curve. The QuantStudio Absolute Q Digital PCR System consists of just one instrument, consolidating all steps required into a single plate and transforming a multi-step multi-instrument workflow into a one-step qPCR-like workflow.

Features

An instrument is only as good as the reagents you use with it. Learn more about our Absolute Q digital PCR master mix, specifically designed for use with the QuantStudio Absolute Q and developed for optimal multiplexing performance and digital PCR sensitivity. TaqMan real-time PCR assays have long been considered the gold standard for genetic analysis. Now, using our decades of experience, unique assay design algorithms, and wet-lab verification process, we have developed a portfolio of equally robust Absolute Q dPCR assays. And, just like our TaqMan Assays, the Absolute Q dPCR assays are backed by a performance guarantee.

Single instrument. Single plate. Simple workflow.

Eliminate cumbersome and challenging sample and reagent preparation steps from your dPCR workflow. The QuantStudio Absolute Q system requires only one hands-on step that takes under five minutes to complete with minimal technical skill.

Industry-leading consistency

Powered by microfluidic array plate (MAP) technology, the QuantStudio Absolute Q system and MAP16 dPCR plates enable exceptional consistency in total micro-chambers analyzed.

Minimize wasted sample volume

Leveraging MAP technology and dead-ended reagent loading, over 95% of your input sample is analyzed per reaction compared to the 25–60% of other dPCR platforms.

Fast time to answer

Avoid wasting precious time moving generating micro-chambers, carrying plates, and reading samples. In under 90 minutes* reagent digitization, thermal cycling, and data collection are integrated into a single system with no manual transfer steps.

*90-minute time includes reagent digitization, thermal cycling, and data collection. The total time may vary based on assay-specific thermal cycling requirements.

  • Key Applications – Rare target quantification, Genotyping, Gene editing, Copy number variation Absolute quantification
  • Research Areas – Oncology, Reproductive health, Infectious disease, Gene Editing & Inherited disease.

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